Method for extracting glutathione from glutathione fermented broth

Abstract

The invention relates to a method to distill glutathione from glutathione fermentation liquid. It uses glutathione fermentation liquid taking centrifuge to gain yeast cell, adjusting pH value, and boiling to distill glutathione in the cell and make the macromolecular substance denaturalization and depositing. Using the positive iron exchange technology to remove small molecule and coloring matter, the white power glutathione that has molecular weight of 307.33, protein content of 23%, and distilling yield 80.5% would be gained. The invention has simple process, high efficient, low cost, high purity, low pollution, etc.

Description

technical field [0001] The invention relates to a method for extracting glutathione from glutathione fermentation liquid, relates to the preparation of glutathione with low protein content and high quality from glutathione fermentation liquid, and belongs to glutathione preparation technology field. Background technique [0002] Glutathione, namely γ-L-glutamyl-L-cysteinyl glycine (glutathione, GSH), is formed by condensation of L-glutamic acid, L-cysteine ​​and glycine through peptide bonds A biologically active tripeptide compound with both γ-glutamyl and sulfhydryl groups. There are two forms of glutathione: reduced GSH and oxidized GSSG. Only GSH is active, and GSSG in organisms needs to be reduced to play its important physiological functions. The reduced glutathione solid is relatively stable, but its aqueous solution is easily oxidized into oxidized glutathione GSSG in the air. GSH is widely distributed in animal, plant and microbial cells in nature. GSH has a var...

AI Technical Summary

Problems solved by technology

[0005] Previous GSH extraction methods generally have disadvantages such as high cost, low extraction rate, poor and unstable quality, and serious environmental pollution, which are difficult to meet the needs of industrial production and practical application.