Method for extracting glutathione from glutathione fermented broth

A technology of glutathione and fermentation broth, which is applied to the preparation methods of peptides, chemical instruments and methods, peptides, etc., can solve the problems of difficulty in adapting to industrial production and practical applications, high cost of GSH extraction methods, and low extraction rates, etc. To achieve the effect of convenient implementation, low production cost and high purity of finished products

Inactive Publication Date: 2006-10-25
JIANGNAN UNIV
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Problems solved by technology

[0005] Previous GSH extraction methods generally have disadvantages such as high cost, low extraction rate, poor and unstable quality, and serious environmental pollution, which are difficult to meet the needs of industrial production and practical application.

Method used

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Embodiment 1

[0017] The glutathione fermented liquid obtained by fermentation, the component analysis result: bacterium 75g / L; GSH content 3.95g / L. The above 2L fermentation broth was centrifuged at 3000rpm for 15min, and the supernatant was discarded to obtain 420g of wet bacteria. Dilute to 5L by adding water to the above wet thallus. Adjust the pH of the diluted bacteria to the range of 1-2 with 2mol / L sulfuric acid. After heating and boiling for 12 minutes, cool to 4°C in an ice-water bath. Put the cooled bacteria mixed liquid directly on the 001×7 cation exchange resin column, the column volume is φ2.6cm×30cm, put the yeast cell liquid containing the bacteria directly on the column, and the column is adsorbed and eluted. At this time, the recovery of GSH is 89.1%, the protein removal rate was 78%, and the eluate was concentrated 5 times. The concentrated glutathione solution concentrated by the adsorption column was concentrated again in vacuum according to the following conditions...

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Abstract

The invention relates to a method to distill glutathione from glutathione fermentation liquid. It uses glutathione fermentation liquid taking centrifuge to gain yeast cell, adjusting pH value, and boiling to distill glutathione in the cell and make the macromolecular substance denaturalization and depositing. Using the positive iron exchange technology to remove small molecule and coloring matter, the white power glutathione that has molecular weight of 307.33, protein content of 23%, and distilling yield 80.5% would be gained. The invention has simple process, high efficient, low cost, high purity, low pollution, etc.

Description

technical field [0001] The invention relates to a method for extracting glutathione from glutathione fermentation liquid, relates to the preparation of glutathione with low protein content and high quality from glutathione fermentation liquid, and belongs to glutathione preparation technology field. Background technique [0002] Glutathione, namely γ-L-glutamyl-L-cysteinyl glycine (glutathione, GSH), is formed by condensation of L-glutamic acid, L-cysteine ​​and glycine through peptide bonds A biologically active tripeptide compound with both γ-glutamyl and sulfhydryl groups. There are two forms of glutathione: reduced GSH and oxidized GSSG. Only GSH is active, and GSSG in organisms needs to be reduced to play its important physiological functions. The reduced glutathione solid is relatively stable, but its aqueous solution is easily oxidized into oxidized glutathione GSSG in the air. GSH is widely distributed in animal, plant and microbial cells in nature. GSH has a var...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K5/037C07K1/18
Inventor 王淼苏晓晋
Owner JIANGNAN UNIV
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