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Pharmaceutical use of heat shock protein 27 for serological identification and liver cancer diagnosis

A technology for heat shock protein and serum preparation, applied in biological testing, scientific instruments, pharmaceutical formulations, etc., can solve problems such as prediction and judgment of liver cancer recurrence and metastasis

Inactive Publication Date: 2006-10-25
ZHONGSHAN HOSPITAL FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, it is mainly relying on regular review of AFP, abnormal prothrombin, fucosyltransferase and B-ultrasound to detect postoperative metastasis and recurrence, but it is still quite difficult to apply it to the prediction and judgment of liver cancer recurrence and metastasis

Method used

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  • Pharmaceutical use of heat shock protein 27 for serological identification and liver cancer diagnosis
  • Pharmaceutical use of heat shock protein 27 for serological identification and liver cancer diagnosis
  • Pharmaceutical use of heat shock protein 27 for serological identification and liver cancer diagnosis

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Experimental program
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Effect test

Embodiment 1

[0024] Using conventional methods in this field, the full protein expression profiles of LCID20 and LCID35 in nude mouse models of human liver cancer with different metastatic potentials were compared and studied: the number of proteins displayed by 2D electrophoresis was as high as 2500-3000, and the analysis and verification of differential proteins were carried out. High expression of HSP27 was found.

Embodiment 2

[0026] Two-dimensional gel electrophoresis (2-DE) was used to separate the proteins of 6 HCC tissues with clinical and pathological metastasis and 6 HCC tissues without metastasis, and the similarities and differences between the 2-DE patterns of the two groups were analyzed using Image Master software 16 protein spots with significant differences were identified by MALDI-TOF-MS and database search. The results showed that these proteins were S100 calcium-binding protein (S100), 27KDa heat shock protein (HSP27), keratin 18 (CK18), etc., which were related to cell movement, signal transduction, energy metabolism, etc. respectively. The expression of HSP27 in 6 cases of metastatic HCC tissues was significantly higher than that in 6 cases of non-metastatic HCC tissues, and the results of 2-DE were further verified by western blotting analysis. There were also differences in mRNA levels between the two groups detected by RT-PCR, but Not significant, immunohistochemical detection s...

Embodiment 3

[0028] Carry out serum proteomics research, on the 2DE map of serum protein after removing high-abundance albumin and globulin, use two-dimensional electrophoresis combined with Maldi-TOF-MS method to compare and study normal people, HBV patients and liver cancer patients Differential expression of proteins, found at least 8 kinds of differential proteins, Transferrin (transferrin, TF), Transthyretin (TTR), α1-antitrypsin (antitrypsin α1), Clusterin (CLU), Haptoglobinchain (hepatoglobulin α2 chain , HP), Ceruloplasmin (CP), Heat shockprotein 27 (heat shock protein 27, HSP27), alpha-fetoprotein (alpha-fetoprotein); semi-quantitatively found that HSP27 is unique to liver cancer.

[0029]The results of Western Blot identification of 60 cases of serum found that no HSP27 was found in normal human serum (0 / 20), and only one case of HBV patients found HSP27 (95%, 1 / 20); all 20 cases of HCC patients had strong HSP27 in serum (100%, 20 / 20). It suggested that HSP27 could be used in th...

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Abstract

The invention relates to the heat shock protein (HSP) 27 medical using. The invention uses the method of protein groupology to take animal mould experiment show that the HSP27 highly expressed. Immunohistochemistry testing result shows HSP27 locating in the liver cell cytoplasm; blood serum protein groupology found that HSP27 is owned by liver cancer; the Western Blot identification result shows HCC patient has strong HSP27(100%, 20 / 20); blood serum double blind testified normal blood serum do not display HSP27(0 / 10), HCC patient positive incidence is 100%(30 / 30). The result shows HSP27 protein could be used as the marking material protein to testing liver cancer. It could be used to make predict and diagnose medicine containing AFP negative HCC and the transformation.

Description

technical field [0001] The invention belongs to the field of biological technology, and relates to a medical use of heat shock protein 27, in particular to the use of heat shock protein 27 in preparing drugs for serological discrimination and diagnosis of liver cancer. Background technique [0002] Malignant tumor is a complex disease involving multiple genes. Its gene expression analysis involves transcriptomic and proteomic methods. Since protein expression or function is regulated at multiple points from transcription to translation, the correlation between the abundance of transcribed mRNA and the amount of translated protein is not obvious. Therefore, proteomics methods are used to study the pathogenesis of tumors as a whole, and to search for tumor diagnosis and Prognostic specific markers and targets for drug therapy have become a recent research focus (Lancet Oncol, 2001, 2(11): 698-704; BMJ. 2000, 320: 424-7). [0003] Tumor markers usually refer to substances uni...

Claims

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Application Information

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IPC IPC(8): G01N33/531A61K39/00G01N33/68
Inventor 刘银坤冯钜涛崔杰锋宋海燕汤钊猷杨芃原
Owner ZHONGSHAN HOSPITAL FUDAN UNIV
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