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Chewing gum compositions comprising monatin and methods for making same

A composition and chewing gum technology, applied in the field of monatin chewing gum composition, can solve problems such as poor sweetness characteristics, unpleasant odor, etc.

Inactive Publication Date: 2006-10-25
CARGILL INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many high-intensity sweeteners have an undesirably off-flavor and / or an unexpected and unsatisfactory sweetness profile

Method used

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  • Chewing gum compositions comprising monatin and methods for making same
  • Chewing gum compositions comprising monatin and methods for making same
  • Chewing gum compositions comprising monatin and methods for making same

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0163] Preparation of Chewing Gum Compositions

[0164] Chewing gum compositions can be prepared using known techniques. In general, chewing gum compositions can be made by sequentially adding the various chewing gum ingredients into any commercially available mixer known in the art (eg, a Sigma paddle mixer). See, eg, US Patent Nos. 5,334,397 and 5,800,848. After the ingredients have been thoroughly mixed, the gum mass is released from the mixer and processed into the desired shape, for example by rolling into sheets and cutting into strips, extruded into blocks or formed into pellets or sheets. Various shapes of chewing and bubble gum are possible, including sticks, cubes, tablets, center filled, balls, fruit, cigarettes, coins, tube gum, and pressed powder gum (compressed powder gum).

[0165]In one embodiment, the gum base is first melted (eg, at a temperature of 80-125°C) and added to a running mixer. Alternatively, the gum base can be melted in a mixer. Color can al...

Embodiment 1

[0170] Cloning and expression of tryptophan aminotransferase

[0171] This example describes the method used to clone tryptophan aminotransferase, which is used to convert tryptophan to indole-3-pyruvate.

[0172] Experiment overview

[0173] Eleven genes encoding transaminases were cloned into E. coli. These genes are Bacillus subtilis (Bacillus subtilis) D-alanine aminotransferase (dat, the Genbank accession numbers of nucleotide sequence and amino acid sequence are Y14082. Rhizobium meliloti) tyrosine aminotransferase (tatA, nucleic acid sequence and amino acid sequence are SEQ ID NOS: 1 and 2), Rhodobacter sphaeroides strain 2.4.1 tyrosine aminotransferase (tatA with homologous determination, The nucleic acid sequence and amino acid sequence are respectively SEQ ID NOS: 3 and 4), Rhodobacter sphaeroides (Rsphaeroides) 35053 tyrosine aminotransferase (determined by homology, the nucleic acid sequence and amino acid sequence are respectively SEQ ID NOS: 5 and 6 ), Leishma...

Embodiment 2

[0177] Conversion of indole-3-lactic acid to indole-3-pyruvate

[0178] Such as figure 1 and 3 As shown, indole-3-lactic acid can be used to produce indole-3-pyruvate. Like the conversion between indole-3-pyruvate and indole-3-lactic acid, the conversion between lactic acid and pyruvate is a reversible reaction. Oxidation of indole-lactic acid can usually be followed due to the high background of indole-3-pyruvate at 340 nm.

[0179] 0.1 mL of standard test mixture containing 100 mM potassium phosphate, pH 8.0, 0.3 mM NAD + , 7 units of lactate dehydrogenase (LDH) (Sigma-L2395, St. Louis, MO) and 2 mM substrate. Assays were repeated 2 times in UV-transparent microtiter plates using a MolecularDevices SpectraMax Plus plate reader. Mix peptide and buffer and pipette to contain indole-3-lactic acid and NAD + After mixing briefly, the absorbance of each well at 340 nm was read at 9 second intervals. The reaction was held at 25°C for 5 minutes. from NAD + Absorbance at 34...

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Abstract

Chewing gum compositions are described that include one or more stereoisomers of monatin, a naturally occurring high-intensity sweetener.

Description

[0001] Cross References to Related Applications [0002] This application claims the benefit of US Provisional Patent Application 60 / 495,191, filed August 14, 2003, which is incorporated by reference in its entirety. technical field [0003] The present invention relates to a novel chewing gum composition containing monatin and a manufacturing method thereof. The composition includes functional chewing gum and bubble gum. The present invention also relates to chewing gum compositions containing specific stereoisomers of monatin, specific mixtures of monatin stereoisomers and / or monatin produced in vivo (e.g. intracellularly) or in vitro by biosynthetic pathways . Background technique [0004] The use of non-calorie high-intensity sweeteners has increased due to concerns about childhood obesity, type 2 diabetes, and related diseases. Therefore, there is a need for a sweetener having a significantly sweeter taste than conventio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23G4/08A23G4/00A23G4/06A23G4/10A23G4/14A23L27/30
CPCA23G4/06A23G4/10A23G4/14A23G4/08A23G4/00
Inventor P·M·西克斯T·W·阿布拉罕D·C·卡梅隆M·J·古森M·G·林德利S·C·麦克法兰J·R·米利斯J·罗萨扎赵利山D·P·维那
Owner CARGILL INC
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