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High-density lipoprotein cholesterol quantitative determining method, reagent and reagent kit

A high-density lipoprotein, quantitative determination technology, applied in the field of quantitative determination of high-density lipoprotein cholesterol, can solve the problem of high price

Active Publication Date: 2007-01-03
BIOSINO BIO TECH & SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] However, the commercially available reagents currently used for direct determination of HDL-C content are all imported products, and the price is relatively high

Method used

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  • High-density lipoprotein cholesterol quantitative determining method, reagent and reagent kit
  • High-density lipoprotein cholesterol quantitative determining method, reagent and reagent kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] One, prepare following reagent I and reagent II of the present invention according to following composition and ratio:

[0038] Reagent I:

[0039] MOPS buffer, pH7.0 30mM

[0040] Sodium dextran sulfate 1g / L

[0041] MgCl 2 ·6H 2 O 2mM

[0042] Color developer TOOS 0.2g / L

[0043] NaN 3 1g / L

[0044] Cyclodextrin sulfate 0.5g / L

[0045] Reagent II:

[0046] MOPS buffer, pH7.0 30mM

[0047] 4-Aminoantipyridine 0.1g / L

[0048] Cholesterol esterase (CEH) 1520u / L

[0049] Cholesterol oxidase (COD) 1070u / L

[0050] Peroxidase (POD) 8000u / L

[0051] Agricultural Emulsion No. 601 5g / L

[0052] NaN 3 1g / L

[0053] 2. Mix 300 μl reagent I and 3 μl serum sample in the sample tube, incubate at 37°C for 5 minutes, use HITACHI7060 automatic biochemical analyzer, measure the absorbance A at the main wavelength of 600nm and the secondary wavelength of 700nm 1 ,...

Embodiment 2

[0067] Using the reagent of Example 1 of the present invention, the HDL-C content in 11 healthy human serum samples was determined according to the method and conditions described in Example 1. For the same sample, use a commercially available precipitation method kit (high-density lipoprotein cholesterol (HDL-C) phosphotungstic acid-magnesium precipitation method kit, batch number: Zhongsheng 19051, produced by Zhongsheng Beikong Biotechnology Co., Ltd.) Comparative quantitative determination, the results are shown in Table 2 below:

[0068]

sample number

HDL-C (mg / dl)

The method of the invention

Precipitation

1

50

52

2

45

42

3

47

50

4

64

68

5

46

48

6

45

44

7

63

67

8

57

60

9

79

80

10

34

32

11

41

44

[0069] Th...

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Abstract

A reagent for the content of the high density lipoprotein cholesterol in the quantitative determining serum sample, consists of the reagent I and reagent II that places respectively, the reagent I contains the heteropolymer anion compound, the divalency cation salt and the decentralized surfactants; the reagent II contains the styrylphenol polyoxyethylene ether. The invention provides a reagent box with the reagent I and the reagent II, and a method for determining the high density lipoprotein cholesterol content.

Description

technical field [0001] The invention relates to a quantitative determination method, reagent and kit for high-density lipoprotein cholesterol (HDL-C). Background technique [0002] Lipids such as cholesterol cooperate with apoproteins in serum to form lipoproteins. Lipoproteins are classified into chylomicrons (CM), very low-density lipoprotein (VLDL), low-density lipoprotein (LDL), high-density lipoprotein (HDL), etc. according to their physical properties. A large-scale epidemiological survey in Frammingham in 1977 showed that the decrease of plasma high-density lipoprotein cholesterol (plasma HDL-C) was the main risk factor for ischemic heart disease. General attention. High-density lipoprotein (HDL) can move the cholesterol in the surrounding tissue to the liver for degradation through the action of enzymes and receptors, and at the same time inhibit cell binding and uptake of low-density lipoprotein cholesterol, and prevent the deposition of cholesterol on the artery ...

Claims

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Application Information

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IPC IPC(8): G01N21/31G01N33/52G01N33/92C12Q1/00
Inventor 张瑛王淑娟
Owner BIOSINO BIO TECH & SCI
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