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Polysaccharide polymer having protein large molecule and its preparation method

A polymer and macromolecule technology, applied in the field of protein macromolecule-loaded polysaccharide-polymer and its preparation

Inactive Publication Date: 2007-01-31
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there has been no report on polysaccharide glass-polymer loaded with protein

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0031] Example 1: Preparation of film-controlled release agent and in vitro release behavior

[0032] 1. Solution configuration:

[0033] 1. Preparation of myoglobin solution: Weigh 20.7 mg of myoglobin, add 2 ml of ultrapure water and vortex to dissolve, and obtain a myoglobin solution with a concentration of about 10 mg / ml.

[0034] 2. Preparation of dextran solution: Weigh 1.0 g of dextran (Mw=64,000-70,000), add ultrapure water to 10.00 g, heat and stir until dissolved. The obtained dextran solution is about 10% by weight.

[0035] 3. Preparation of PEG solution: Weigh 1.0 g of dextran (Mw=8,000), add ultrapure water to 10.00 g, heat and stir until dissolved. The obtained dextran solution is about 10% by weight.

[0036] 2. Preparation of model protein-dextran particles:

[0037] 1. Preparation of myoglobin dextran microparticles: Weigh 0.50g, 0.16g and 1.00g of myoglobin solution, dextran solution and PEG solution respectively (note two parts), and use homogenate under ...

example 2

[0045] Example 2: Preparation and in vitro release behavior of stent coating model controlled release agent

[0046] 1. Preparation of model protein-dextran particles according to Example 1

[0047] 2. Model protein (myoglobin) coating preparation:

[0048] 1:5 coating preparation: Weigh 15.1mg of PLGA (50 / 502A) and 1ml of dichloromethane, vortex to dissolve. Add 700ul of shaken myoglobin dextran particle suspension, and vortex to make the dispersion even. The above solution was sprayed onto a 316L stainless steel metal sheet with an airbrush, and the prepared metal sheet was decompressed to remove dichloromethane.

[0049] 1:10 coating preparation: Weigh 29.9 mg of PLGA (50 / 502A), and prepare the coating in the same way as above.

[0050] 1:15 coating preparation: Weigh 45.6 mg of PLGA (50 / 502A), and prepare the coating in the same way as above.

[0051] Blank coating preparation: Weigh an appropriate amount of PLGA (50 / 502A) and 1ml of dichloromethane as the above three ...

example 3

[0054] Example 3: Preparation and in vitro release behavior of heart stent coating controlled release agent

[0055] 1. Preparation of model protein-dextran microparticles according to Example 1;

[0056] Two, heart stent coating is prepared according to example 1

[0057] 1:5 coating preparation: Weigh 15.1mg of PLGA (75 / 253A) and 1ml of dichloromethane, vortex to dissolve. Add 700ul of shaken myoglobin dextran particle suspension, and vortex to make the dispersion even. The above solution was sprayed onto the surface of the heart stent with a spray pen, and the prepared heart stent was decompressed to remove the methylene chloride. The surface of the heart stent can be clearly seen through the scanning electron microscope that the surface is smooth and flat without burrs, and the stent coating is suitable for clinical application.

[0058] 3. Release test of the cardiac stent coating: the release solution was measured according to Example 1; the in vitro release curve sho...

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Abstract

The present invention relates to a protein macromolecule bearing polysaccharide-polymer in the field of biological technology and its preparation method. According to the concrete requirement the different polysaccharides, proteins, polymers and protective reagent can be selected, in which polysaccharide content is 0.01%-50%, protein content is 0.01%-50%, polymer content is 20%-80%, reagent content for protecting protein is 0%-10%. Its preparation method includes the following steps: (1), adding protein into polysaccharide solution; (2), mixing protein-polysaccharide and polyethylene glycol solution; (3), freeze-drying the formed mixed solution or water phase-water phase emulsion; (4), adding the obtained freeze-dried powder into organic solution of soluble polyethylene glycol to wash and remove polyethylene glycol so as to obtain particles; and (5), adding the obtained particles into the solution of liposoluble polymer and uniformly mixing them, making the emulsion be formed for standing-by. Said invention can be used for making protein medicine intervenient therapy.

Description

technical field [0001] The invention relates to a polymer in the field of biotechnology and a preparation method thereof. In particular, a polysaccharide-polymer loaded with protein macromolecules and a preparation method thereof. Background technique [0002] Biocompatible, degradable polymers have been widely used in disease treatment and other related fields. Combining such materials with biotechnological drugs that have developed rapidly in recent years has attractive application prospects, but at the same time encounters the technical bottleneck of how to maintain relatively fragile biotechnological drugs in such materials with low stability. Compared with chemical small molecule drugs, protein macromolecules have stronger specificity and better selectivity, but their fragile and variable structures have become a problem to be solved in the preparation of biomedical materials. In particular, biomedical materials including tissue engineering materials often need to be ...

Claims

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Application Information

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IPC IPC(8): C08L67/04C08L83/04C08L27/18C08L23/00C08L25/06C08L77/00C08L5/02C08L3/02C08L1/00C08L89/00A61K31/715A61K38/00
Inventor 金拓袁伟恩吴飞
Owner SHANGHAI JIAO TONG UNIV
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