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Separation identification method of pig pankrin inhibition protein gene monomer type

A calpain and inhibitory protein technology, applied in the field of livestock molecular biology, can solve the problems of lower consumer satisfaction, poor palatability, and poor quality of pork

Inactive Publication Date: 2007-01-31
SHANGHAI JIAO TONG UNIV
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  • Claims
  • Application Information

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Problems solved by technology

On the other hand, with the pig industry's genetic selection to improve the growth rate and feed efficiency of meat pigs, the production level of meat pigs has been improved, but at the same time, the quality of pork has become coarse and old, and the palatability is poor, which reduces consumer expectations. meat quality satisfaction

Method used

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Embodiment

[0023] (1) Pig ear tissue was collected, and genomic DNA was extracted using a conventional phenol / chloroform extraction method, and the DNA sample was diluted to 100 ng / μL for later use. Among them, there are 125 experimental pigs, which are PIC five-line hybrid commercial pigs, provided and raised by Shanghai Nongshang Meat Food Company. When the average body weight reaches 90 kg, they are slaughtered intensively.

[0024] (2) For the single nucleotide polymorphism sites 200A / G and 1187C / T of the calpain gene, design specific primers for the calpain gene and perform a PCR reaction, wherein the nucleotide numbers are based on the GenBank index No.: AY594692. The primers refer to the porcine calpain gene protein coding sequence (GenBank Acc №: M20160), and use the Primer Premier 5.0 software to design primers by yourself. The primers were synthesized by Shanghai Gene Base Technology Co., Ltd. The gene-specific primers are 5'-AAAGCCTACCCACGCACT-3' and 5-GCAGATACCACCAGTAACAGA...

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Abstract

The present invention relates to a separation and identification method of swine calpastatin gene monomer type, belonging to the field of livestock molecular biology technology. Said method includes the following steps: extracting genome DNA of swine; PCR amplification of calpastatin gene; pleiomorphism detection of amplification product; formation of monomer type and correlation analysis of said monomer type and pork quality character. Said invention is characterized by that two new swine calpastatin gene monomer types of H2121 and H1211 are separated and identified.

Description

technical field [0001] The invention relates to a method for isolating and identifying porcine calpain inhibitor gene haplotypes. Two new porcine calpain inhibitor gene haplotypes are constructed by this method, and their characteristics significantly related to pork meat quality traits are utilized. It provides a new molecular marker method for marker-assisted selection and mating of pigs. The invention belongs to the technical field of livestock molecular biology. Background technique [0002] With the improvement of living standards, people have higher requirements for food. As an important part of people's diet, pork is the focus of people's improvement in recent years. Lean and tender, juicy and delicious pork is very popular among consumers in the market. On the other hand, with the pig industry's genetic selection to improve the growth rate and feed efficiency of meat pigs, the production level of meat pigs has been improved, but at the same time, the quality of po...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/10
Inventor 潘玉春王起山孙立彬李婧白春艳
Owner SHANGHAI JIAO TONG UNIV