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Composite protein precipitator

A technology for compounding proteins and precipitants, applied in the field of protein precipitants, can solve the problems of large sample loss, poor extraction and separation effect, and complicated operation.

Active Publication Date: 2010-05-12
HARBIN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to solve the low protein precipitation rate of the existing single component protein precipitant, the poor extraction and separation effect, the excessive acidity of 10% trichloroacetic acid and 6% perchloric acid are too strong to the chromatographic column, the cost is high and the The liquid-liquid extraction method is used to extract the defects of long time-consuming, cumbersome operation and large sample loss of drugs in biological samples, thus providing a composite protein precipitant

Method used

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Experimental program
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Effect test

specific Embodiment approach 1

[0005] Specific embodiment 1: In this embodiment, the composite protein precipitation agent is from 1 to 1.5 parts by weight of analytically pure 5-sulfosalicylic acid, 75 to 85 parts is analytically pure methanol, and 15 to 25 parts by weight. Made of distilled water.

[0006] In this embodiment, 5-sulfosalicylic acid whose purity is analytically pure, methanol whose purity is analytically pure, and distilled water are mixed and dissolved in parts by weight and then used.

[0007] The composite protein precipitant of this embodiment is applied to the pretreatment of biological samples of cardiovascular drugs such as Celilol hydrochloride, so as to perform reversed-phase high performance liquid chromatography analysis. Processing steps of biological samples (plasma):

[0008] (1) Mix and shake the biological sample and the composite protein precipitation agent of this embodiment at a volume ratio of 1:1;

[0009] (2) Centrifugation, protein precipitation, and take the supernatant for...

specific Embodiment approach 2

[0014] Embodiment 2: The difference between this embodiment and Embodiment 1 is that the composite protein precipitation agent ranges from 1.1 to 1.4 parts by weight to analytically pure 5-sulfosalicylic acid, and from 76 to 84 parts by weight. It is made of analytically pure methanol and 16 to 24 parts of distilled water. The other steps are the same as the first embodiment.

specific Embodiment approach 3

[0015] Specific embodiment three: The difference between this embodiment and the specific embodiment one is that the composite protein precipitation agent has a purity of 1.2 to 1.3 parts by weight to analytically pure 5-sulfosalicylic acid, and a purity of 78 to 82 parts. It is made of analytically pure methanol and 18-22 parts of distilled water. The other steps are the same as the first embodiment.

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Abstract

the invention discloses a compound protein precipitant, which is characterized by the following: possessing 1-1.5% 5-sulfosalicylic acid, 75-85% carbinol and 15-25% distilled water; making rate of deposition over 99%; damaging little for chromatographic column through pH at weak acid.

Description

Technical field [0001] The invention relates to a protein precipitation agent. Background technique [0002] The existing organic solvent protein precipitants, acidic or basic protein precipitants and heavy metal protein precipitants are mostly single components, the protein precipitation rate is low, and the extraction and separation effect is poor. Although 10% trichloroacetic acid and 6% perchloric acid have a high protein precipitation rate, the protein precipitation rate is 98.5%-99.5% in the case of the same volume as the sample, but there is excessive acidity and damage to the column The problem of large and high cost; and the use of liquid-liquid extraction method to extract drugs from biological samples is time-consuming, cumbersome to operate, and large loss of drug components in the samples, which greatly affects the efficiency and accuracy of in vivo drug analysis. Meet the needs of rapid and accurate provision of experimental data. Summary of the invention [0003] ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K1/30
Inventor 杜智敏张波
Owner HARBIN MEDICAL UNIVERSITY
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