The invention provides a seedling raising method of pearl guava, and belongs to the technical field of agricultural product processing. According to the method, fertilizer is applied to a seed tree before girdling, and the fertilizer includes root fertilizer applied 6 months before girdling, leaf surface fertilizer applied 4 months before girdling, compound sodium nitrophenolate sprayed to branches and leaves 3 months before girdling, and paclobutrazol sprayed to branches and leaves 2 months before girdling; a special rooting substrate special for girdling notches is further adopted and is prepared from, by weight, 52-70 parts of soy sauce residues, 80-100 parts of moss, 100-120 parts of yellow mud, 20-30 parts of modified plant ash, 10-15 parts of powdered rock phosphate, 0.002-0.003 part of ethylene chlorohydrin, 0.001-0.002 part of naphthylacetic acid and 0.001-0.002 part of fulvic acid. The steps are matched with one another and influence one another, and therefore the damage of bending pruning on the seed tree is reduced, the bending pruning seedling raising is shortened, roots is robust, and the plantation survival rate is high.