Method of synchronous distinguishing newcastle disease virus and vaccine virus and identifying virulence and genotype
A newcastle disease virus and vaccine virus technology, applied in the field of automated analysis software, can solve the problems of small number of verification samples, insufficient theoretical foundation, and difficult application
Inactive Publication Date: 2007-03-14
HUZHOU TEACHERS COLLEGE
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AI-Extracted Technical Summary
Problems solved by technology
The present invention discloses molecular biological method and automatic analysis software for distinguishing wild virus strain and vaccine virus strain of new castle disease virus (NDV), and identifying the toxicity and genotype simultaneously. The NDV is RT-PCR amplified with one pair of specific degenerate primers to obtain one multipurpose NDV segment for direct sequencing of the PCR product. One automatic analysis software is composed for comparing the homology of the sequence to the standard vaccine sequence so as to distinguish wild virus strain and vaccine virus strain, analyze the toxicity by means of the F gene cracking site and judge the genotype through developing tree analysis. The present invention has short detecting period, reliable detection result and capacity of obtaining several detection results simultaneously, and is suitable for use in fowl product inspector and quarantiner.
Direct sequencingDegenerate primer +11
- Experimental program(1)
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