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Total monoclonal antibody of AIDS-resisting surface glucoprotein and its prepn process

A surface glycoprotein and monoclonal antibody technology, applied in the field of biomedicine, can solve problems such as inability to take oral administration, instability in case of acid, and small T-20 molecules

Inactive Publication Date: 2007-03-21
上海晨健抗体组药物有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The drug T-20 targeting gp41 is already on the market, but there are many problems in patients with long-term use, such as T-20 molecule is too small, it is easy to develop resistance; it is unstable in acid and cannot be taken orally; it needs intravenous injection every day wait twice

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  • Total monoclonal antibody of AIDS-resisting surface glucoprotein and its prepn process
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  • Total monoclonal antibody of AIDS-resisting surface glucoprotein and its prepn process

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Embodiment Construction

[0036] The invention provides a fully human monoclonal antibody against HIV surface glycoprotein gp41. The antibody has the heavy chain reported in Sequence 9 and the light chain reported in Sequence 10, has no mouse-derived heterologous protein, is completely consistent with the antibody naturally produced in the human body, and has the optimal specificity that has been selected and screened by the entire immune system of the human body Affinity and bonding. The antibody can be used for clinical diagnosis and treatment of AIDS.

[0037] At the same time, the present invention also provides a method for preparing the antibody, and at least the steps included in the method will be described below through a preferred embodiment.

[0038] (a) Screen patients with high titer gp41 antibody, and isolate peripheral blood B cells from AIDS patients.

[0039] Select 100 volunteers, all of whom are AIDS patients, remove 5ml of venous blood each, separate the serum, store at -20°C, and...

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Abstract

The present invention reveals one kind of total human monoclonal antibody of AIDS-resisting surface glucoprotein gp41 and its preparation process. The antibody is prepared through human-human hybridoma technology and other monoclonal antibody technology, and has heavy chain and light chain as shown and no mouse originated foreign protein, is identical with natural human antibody, and possesses optimized specific affinity and binding power. It is prepared through the following steps: screening AIDS patient with high titer gp41 antibody and separating his peripheral blood B cell; fusing myeloma Karpas707H and peripheral blood B cell to generate hybridoma; screening hybridoma generating high gp41 affinity antibody; preparing and purifying antibody; extracting cell total mRNA and obtaining antibody series with common primer; and loading to T and sequencing.

Description

technical field [0001] The invention belongs to the field of biomedicine, and relates to a fully human monoclonal antibody against AIDS surface glycoprotein and a preparation method thereof, in particular to a fully human monoclonal antibody against AIDS surface glycoprotein gp41 and a preparation method thereof. Background technique [0002] Since the first case of AIDS was confirmed by the US Centers for Disease Control (CDC) in June 1981, AIDS has been rampant all over the world, seriously threatening human health and survival. According to the latest statistics from the United Nations Program on AIDS and the World Health Organization, by the end of 2001, a total of 5 million people worldwide were infected with HIV, and there were at least 40 million HIV carriers, of whom 2.7 million were children under the age of 15. Globally, AIDS is one of the ten leading causes of death. According to the current development trend, in the near future, AIDS will replace some common cau...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/28C12N5/22
Inventor 倪健杨子义罗鹏杨武剑
Owner 上海晨健抗体组药物有限公司
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