Biological process of converting sugar-containing solution into perfume for cigarette
A technology for biotransformation and tobacco flavors, which is applied in the fields of essential oils/spice, tobacco, and applications, and can solve problems such as high equipment requirements, unsatisfactory effects, and potential safety hazards.
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Embodiment 1
[0023] ① Firstly, the Saccharomyces cerevisiae strain A cultured on the slant 1 , A 2 , A 3 , A 4 , A 5 Inoculate 0.5% by weight into 100ml of culture medium placed in Erlenmeyer flasks, culture at 30°C and 200rpm on a shaker for at least 24 hours to obtain a well-grown culture solution, and then centrifuge the above culture solution at 2356×g for at least 10 minutes Collect the bacterial cells, then wash the bacterial cells at least twice with deionized water and centrifuge twice to remove the residual medium as much as possible, then add water to prepare the yeast cell seed solution, and the concentration of the bacterial cells is 200,000 / cm 3 , to obtain five yeast cell seed solutions B 1 , B 2 , B 3 , B 4 , B 5 (for the next step). The medium mentioned above is a kind of sugar source 1.5wt%, peptone 0.5wt%, yeast extract 0.5wt%, KH 2 PO 4 0.2wt%, NaCl 0.1wt%, MgSO 4 0.05 wt%, and the balance is a solution composed of water; the sugar source, also known as the...
Embodiment 2
[0027] Yeast seed solution B 6 , B 7 , B 8 , B 9 and B 10 The cultivation and biotransformation of tobacco flavors.
[0028] ①Except for strain A 6 、A 7 、A 8 、A 9 or A 10 Outside, all the other culture conditions and treatment methods are all the same as 1. in embodiment 1, finally obtain respectively five yeast cell seed liquids: B 6 , B 7 , B 8 , B 9 , B 10 , for the next step.
[0029] ②Biotransformation of the liquid to be transformed: inoculate the seed liquid of yeast cells obtained above into the liquid to be transformed by 10% by volume, and place it in a conventional 5L fermenter with stirring (sample loading is 3L), Biotransform at a temperature of 20-40° C. for at least 72 hours to obtain a fermentation broth. Wherein said concentration and composition of the solution to be converted are all the same as those of 2. in Example 1.
[0030] ③ Centrifuge the fermented liquid obtained above to remove the thallus to obtain the supernatant, and then sterili...
Embodiment 3
[0032] Get the saccharomyces seed liquid B of gained in embodiment 1 1. 1 , B 2 , B 3 , B 4 or B 5 , respectively inoculated into the liquid to be transformed according to the volume ratio of 10% (v / v), placed in a stirred 5L fermenter (sample loading is 3L), at a temperature of 30°C, biotransformed for at least 72h to obtain a fermented liquid . Wherein said liquid to be converted is an aqueous solution containing 15% of maltose or cellobiose in terms of mass concentration.
[0033] After putting the fermented liquid obtained above into the tank, centrifuge them separately to remove the bacteria to obtain the supernatant, and then sterilize the supernatant by microwave for at least 1 minute to obtain five tobacco flavoring products C according to the present invention. 1a 、C 2a 、C 3a 、C 4a 、C 5a . After testing, it was found that although the above products were biotransformed by different yeasts, their aroma components and solid sugars were basically the same, so t...
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