Biological process of converting sugar-containing solution into perfume for cigarette

A technology for biotransformation and tobacco flavors, which is applied in the fields of essential oils/spice, tobacco, and applications, and can solve problems such as high equipment requirements, unsatisfactory effects, and potential safety hazards.

Inactive Publication Date: 2007-03-21
HUABAO FLAVOURS & FRAGRANCES CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are usually two ways to add sugar in cigarettes. One is to directly add some simple sugars, such as glucose and fructose. Although this method can improve the taste of cigarettes to a certain extent, the effect has been proved to be unsatisfactory. Besides taste, it has almost no other effect, and sugar is one of the important factors to produce tar. Therefore, effectively controlling the sugar content of tobacco leaves in the leaf group formula is one of the main contents to be considered in the design of low-tar cigarettes; One method is to add the Maillard reaction product of sugar (refer to: Tobacco Science and Technology, 1994 (6), 21-24, the Maillard product of sugar), which can improve the taste of cigarettes and also to a certain extent Increase the concentration of cigarette aroma, so it is generally recognized that the Maillard product of adding sugar is better than adding monosaccharide directly, but this method also has a big disadvantage, that is, the Maillard reaction of sugar needs to be carried out under high temperature and high pressure, not only The requirements for equipment are relatively high, there are potential safety hazards, and the energy consumption is relatively large, so the above two methods are not ideal.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] ① Firstly, the Saccharomyces cerevisiae strain A cultured on the slant 1 , A 2 , A 3 , A 4 , A 5 Inoculate 0.5% by weight into 100ml of culture medium placed in Erlenmeyer flasks, culture at 30°C and 200rpm on a shaker for at least 24 hours to obtain a well-grown culture solution, and then centrifuge the above culture solution at 2356×g for at least 10 minutes Collect the bacterial cells, then wash the bacterial cells at least twice with deionized water and centrifuge twice to remove the residual medium as much as possible, then add water to prepare the yeast cell seed solution, and the concentration of the bacterial cells is 200,000 / cm 3 , to obtain five yeast cell seed solutions B 1 , B 2 , B 3 , B 4 , B 5 (for the next step). The medium mentioned above is a kind of sugar source 1.5wt%, peptone 0.5wt%, yeast extract 0.5wt%, KH 2 PO 4 0.2wt%, NaCl 0.1wt%, MgSO 4 0.05 wt%, and the balance is a solution composed of water; the sugar source, also known as the...

Embodiment 2

[0027] Yeast seed solution B 6 , B 7 , B 8 , B 9 and B 10 The cultivation and biotransformation of tobacco flavors.

[0028] ①Except for strain A 6 、A 7 、A 8 、A 9 or A 10 Outside, all the other culture conditions and treatment methods are all the same as 1. in embodiment 1, finally obtain respectively five yeast cell seed liquids: B 6 , B 7 , B 8 , B 9 , B 10 , for the next step.

[0029] ②Biotransformation of the liquid to be transformed: inoculate the seed liquid of yeast cells obtained above into the liquid to be transformed by 10% by volume, and place it in a conventional 5L fermenter with stirring (sample loading is 3L), Biotransform at a temperature of 20-40° C. for at least 72 hours to obtain a fermentation broth. Wherein said concentration and composition of the solution to be converted are all the same as those of 2. in Example 1.

[0030] ③ Centrifuge the fermented liquid obtained above to remove the thallus to obtain the supernatant, and then sterili...

Embodiment 3

[0032] Get the saccharomyces seed liquid B of gained in embodiment 1 1. 1 , B 2 , B 3 , B 4 or B 5 , respectively inoculated into the liquid to be transformed according to the volume ratio of 10% (v / v), placed in a stirred 5L fermenter (sample loading is 3L), at a temperature of 30°C, biotransformed for at least 72h to obtain a fermented liquid . Wherein said liquid to be converted is an aqueous solution containing 15% of maltose or cellobiose in terms of mass concentration.

[0033] After putting the fermented liquid obtained above into the tank, centrifuge them separately to remove the bacteria to obtain the supernatant, and then sterilize the supernatant by microwave for at least 1 minute to obtain five tobacco flavoring products C according to the present invention. 1a 、C 2a 、C 3a 、C 4a 、C 5a . After testing, it was found that although the above products were biotransformed by different yeasts, their aroma components and solid sugars were basically the same, so t...

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Abstract

The present invention discloses biological process of converting sugar-containing solution into perfume for cigarette. Saccharomyces cerevisiae is utilized in converting sugar into flavor enhancing perfume for cigarette. The present invention provides one way for applying sugar in cigarette making to raise the quality of cigarette product and promote the development of cigarette perfume industry.

Description

technical field [0001] The invention relates to a method for biotransformation to produce tobacco flavor, in particular to a method for biotransformation into flavor-increasing tobacco flavor by using a sugar solution as a raw material. Background technique [0002] Biotransformation is one of the three methods for producing spices. Compared with natural extraction and chemical synthesis, biotransformation has unique advantages—simple process, mild conditions, and environmental friendliness. Therefore, how to use biotransformation methods to efficiently obtain high-quality tobacco flavors has become a current research hotspot. [0003] Sugar substances are used as flavoring agents for tobacco products. An appropriate amount of sugar content can make cigarettes taste mellow and less irritating, because the pyrolysis products of sugar are acidic. There are usually two ways to add sugar in cigarettes. One is to directly add some simple sugars, su...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B9/00A24B15/00
Inventor 黄汉荣罗昌荣穆海菠
Owner HUABAO FLAVOURS & FRAGRANCES CO LTD
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