Unlock instant, AI-driven research and patent intelligence for your innovation.

Diabetic cardiovascular pathological change related gene and its use

An amino acid, cardiomyocyte technology, applied in genetic engineering, plant genetic improvement, application, etc.

Inactive Publication Date: 2007-04-18
SHANGHAI INST FOR ENDOCRINE & METABOLIC DISEASES
View PDF1 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, so far, little is known about the genes associated with diabetic cardiovascular disease. Therefore, there is an urgent need to find and develop new diabetic cardiovascular disease-related genes and their encoded proteins.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Diabetic cardiovascular pathological change related gene and its use
  • Diabetic cardiovascular pathological change related gene and its use
  • Diabetic cardiovascular pathological change related gene and its use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0117] Embodiment 1. Preparation of diabetic rat model

[0118] 1.1 Animals and materials

[0119] 100 clean-grade male SD (Sprague-Dawley) rats, 2 months old, weighing (250±20) g, were purchased from BK Company, animal certificate number: Shanghai Dong 152; purchased from STZ (streptozotocin) Provided by Sigma Company; blood glucose, triglyceride, cholesterol and other biochemical detection kits were purchased from Mingdian Bioengineering Co., Ltd.; neutral unimodal pure insulin was purchased from Wanbang Biochemical Pharmaceutical Co., Ltd.; insulin primary antibody and related immunohistochemical reagents were purchased from From Maixin Company; Insulin Radioimmunization Kit for Rats was purchased from Linco Company.

[0120] Dietary ingredients: the average calorie of common feed is 3.9cal / g, of which carbohydrates account for 60%, protein accounts for 22%, fat accounts for 10% (mainly soybean oil), other ingredients including cellulose account for 8%; high-fat feed avera...

Embodiment 2

[0140] Example 2 Screening of genes related to early cardiomyopathy in rats with type 2 diabetes

[0141] 2.1 Materials

[0142] 2.1.1 Main instruments and equipment

[0143] Genomyx LRS fluorescence differential display analysis system (Beckman), the system includes (1) Genomyx LR electrophoresis instrument, including automatic gel drying device (2) Genomyx SC scanner (3) analysis software system includes Acquire SC, Clarity SC, ExtendLRS, Virtual (4) Glue cutting workstations such as Grid System. Spectrophotometer (Beckman DU650), 9700 PCR instrument (PE), Fluor-s TM multilmager gel image analyzer (Bia-RAD), low-temperature high-speed centrifuge (Beckman), hybridization oven (Robbins Scientific 2000).

[0144] 2.1.2 Main reagents

[0145] The HIEROGLPH mRNA Profile Kit (Beckman) includes: (1) 12 T7 (dT12) anchor primers (Anchored Primer, AP) with 3' end unlabeled fluorescence (2) 20 5'M13r random primers (Arbitrary Primer, ARP ). Fluoro-DDRT-PCR kit (Beckman) includes:...

Embodiment 3

[0212] Embodiment 3, neonatal rat primary cardiomyocyte isolation and culture

[0213] Take 12 newborn S-D rats 1-2 days old, soak them in 75% alcohol for 1 minute, and then take them out. They were wiped dry with alcohol cotton balls and executed by breaking the cervical vertebrae. A two-centimeter incision was made obliquely upward on the chest wall at the junction of the xiphoid process and the left 5th-6th rib to expose the apex of the heart, and the ventricle was quickly cut off and soaked in D-Hanks solution. After obtaining all twelve hearts, they were washed 6 times in ice D-Hanks solution to wash away the congestion in the ventricular cavity and destroy the endothelial cells of the ventricle. Use ophthalmic scissors to cut the above myocardial tissue into 1mm×1mm pieces, transfer it into a 50ml glass Erlenmeyer flask, continue to wash with D-Hanks solution, wait for the tissue to settle, and suck off the supernatant. Repeat twice. Add 0.125% trypsin + 0.01% EDTA (d...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

This invention provides a new diabetic cardioangiopathy GAP-associated protein GAP-RDCR2 protein, polyribonucleotide coding RDCR2 protein and the method of generating this RDCR2 protein by recombination technology.This invention also discloses the use of polyribonucleotide coding this RDCR2 protein. RDCR2 protein has the function of inducing apoptosis by priming cadiocyte thread granules under high glucose environment.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular, the invention relates to a new polynucleotide encoding rat diabetic cardiovascular disease-related protein RDCR2, and a polypeptide encoded by the polynucleotide. The present invention also relates to the use and preparation of such polynucleotides and polypeptides. Background technique [0002] Type 2 diabetic cardiovascular disease is not only the main cause of death of patients, but also has a higher incidence, faster development process, and serious condition than the general population. It is necessary to strengthen the research on its pathogenesis. [0003] Although people have recognized the interaction of protein non-enzymatic glycation, excessive free radical production, polyol metabolism, lipoprotein polymorphism, cytokines and growth factors in the pathogenesis process, the research progress in this area There are two main reasons. One is the difficulty in obtaining clinica...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/47C12N15/12C12N15/63C12P21/02G01N33/68C12N5/10
Inventor 罗敏
Owner SHANGHAI INST FOR ENDOCRINE & METABOLIC DISEASES