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A seedling environment training method for ginger tissue cultured test-tube seedling

A technology for test-tube seedlings and seedling hardening, applied in horticultural methods, botany equipment and methods, plant regeneration, etc., can solve the problems of unfavorable ginger rhizome expansion, low survival rate of hardened seedlings, and slow seedling return speed, etc., and achieve good implementation effect , good promotion and application, and easy to operate

Inactive Publication Date: 2011-03-16
徐坤 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Due to the tenderness of the ginger tissue culture test-tube plantlets, weak root system, poor regeneration ability, and the large difference between the microenvironment in the bottle and the field environment before and after transplanting the test-tube plantlets, the survival rate of the test-tube plantlets transplanted in the field is often low. seedlings, the speed of returning seedlings is slow, which is not conducive to the expansion of ginger rhizomes
According to non-patent literature " Anhui Agricultural Science " (28 volumes 4 phases in 2000) " Seedling Hardening Technology of Tissue Cultured Seedlings " report, seedling hardening substrate and environmental condition are the core issues of seedling hardening technology, and whether the hardening technology is passed is the limit. The key to whether the industrialized seedling production line can run, and the survival rate of hardened seedlings will ultimately affect the production scale of the product

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Ginger tissue culture test-tube seedling hardening technology in a bottle: The time of hardening seedlings in the bottle, the light intensity of hardening, and the temperature of hardening have a significant impact on the survival rate of ginger test-tube seedlings transplanted from the bottle to the seedling tray (Table 1) . In the stage of hardening seedlings in test tubes and bottles, the light intensity of 15-25klx and the temperature of 25-27°C are the most suitable. The light intensity of 4-10klx is too weak, and the nutrition produced by the leaves is insufficient. It is difficult to produce new roots after transplanting, and the survival rate is low; The light of 35-45klx is too strong, and the leaves of the test-tube plantlets are easily burned and dried up, which leads to a low survival rate of the test-tube plantlets after transplanting. However, the time of hardening seedlings in the bottle within 3-15 days had no significant effect on the survival rate of t...

Embodiment 2

[0028] The seedling hardening effect of different formula hardening substrates: under the same nutrient composition and the same seedling hardening environmental conditions, different seedling hardening matrix formulas have significantly different hardening effects (Table 2), wherein grass carbon: vermiculite: clay = 1:1:1 substrate ratio, the highest survival rate of seedling hardening, up to 87.2%, and the seedling quality is better, the average plant height is 15.8cm, 4.8 new roots, 3.2 new leaves. However, the seedling hardening effect of simply using soil, vermiculite and perlite is not as good as the former.

[0029] Table 2 The impact of matrix formula on the survival rate (%) of ginger tissue culture test-tube seedlings in bottle

[0030]

Matrix formulation

Add ingredients

Survival rate of hardened seedlings

(%)

seedling height

(cm)

new leaves

slices

happened new

Number of roots

soil

Ver...

Embodiment 3

[0034] Effects of ambient temperature and light intensity on the survival rate of ginger test-tube seedlings: under the same conditions, different light intensities or different temperatures have different effects on the hardening of the external medium (Table 3). According to statistics on the investigation results of 1000 test-tube seedlings transplanted for 30 days, it is found that the light intensity of matrix hardening at noon is about 35klx, and the temperature of matrix hardening is preferably 24-28°C, and the transplanted test-tube seedlings survive. The rates were 87.4% and 86.9%, and the seedlings had more new leaves and new roots, the quality of the seedlings was better, and the survival rate of the transplanted field was higher.

[0035] Table 3 The impact of the ambient temperature and light intensity on the survival rate (%) of the ginger test-tube plantlets 1)

[0036]

light intensity at noon 2) (klx)

temperature 3) (℃)

15

25...

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Abstract

The invention relates to a ginger cultivate seeding method. Wherein, it comprises bottle seeding and external substrate seeding processor; and it is characterized in that: a, bottle seeding that putting ginger tube in the sealed condition with 20-28Deg. C, 15-25klx light, 12h / d lighting time, and 80% RH, opening bottle cover, to apply the condition for 3-5d; b, external substrate seeding that taking out the seed in step a, planting it at the substrate bed in seeding room, while the substrate comprises grass carbon, vermiculite and at 1:1:1 ratio; and the temperature of seeding room is 24-28Deg. C at day and 16-20Deg. C at night, with 25-40klx light at afternoon, 70-85% RH, and 25-30d, then to be planted into large field.

Description

1. Technical field [0001] The technical invention relates to a method for hardening ginger tissue-cultured test-tube seedlings, which is specially used for hardening and transplanting ginger tissue-cultured test-tube seedlings, and belongs to the technical field of vegetable seedling breeding. 2. Background technology [0002] Ginger (Zingiber officiale Roscoe) is a vegetatively propagated crop with rhizomes as product organs and reproductive organs. Since its growth and development have not undergone sexual generations, viruses are easy to accumulate in the body, resulting in growth potential decline, species degradation, and stress resistance. The aggravation of diseases and insect pests and the decline of yield and quality have seriously restricted the competitiveness of ginger products in the international market. Therefore, it is one of the important technical measures to increase ginger yield and improve ginger quality through shoot tip tissue culture, breeding tissue-...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G7/00C12N5/04
Inventor 徐坤郑永强
Owner 徐坤