Direct assay of skin cholesterol in skin samples removed by tape stripping

A cholesterol and skin technology, applied in the direction of material inspection products, inoculation and ovulation diagnosis, microbial measurement/inspection, etc.

Inactive Publication Date: 2007-05-09
麦拉克林有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is not a simple and rapid method for the determination of cholesterol in large samples

Method used

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  • Direct assay of skin cholesterol in skin samples removed by tape stripping
  • Direct assay of skin cholesterol in skin samples removed by tape stripping
  • Direct assay of skin cholesterol in skin samples removed by tape stripping

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0112] The present invention utilizes double-coated pressure sensitive medical tape sold by Adhesive Research, Inc. with the upper sampling side covered with a protective release liner. Cut a 1 inch square piece of tape and attach this piece of tape to one end of a 1 inch by 3 inch piece of plastic (white polystyrene plastic) using the exposed lower adhesive side of the tape, leaving a 1 inch by 2 inch The uncovered plastic acts as a handle for applying the tape to the skin and marking samples.

[0113] To obtain a skin sample, the protective liner is removed and the exposed adhesive area is taped to clean, dry skin. Apply pressure to the back of the plastic over the adhesion area to allow the adhesive to make full contact with the skin. The plastic piece with the adhesive tape and the cuticle sample was then peeled off from the skin.

[0114]Cut the sample into 4 pieces of equal size and measure the area of ​​each piece in microinches x microinches. Place one piece into a ...

Embodiment 2

[0116] The sampling device shown in Figure 1 was used. The sampling device, indicated generally by the reference numeral 10, is made of plastic (polystyrene) and comprises a sampling member 12 connected to a closure member 14 by an integral hinge 16 . The closure 14 has a frame 18 and four pegs 20 that lock into side grooves 22 and four holes 24 of the sampling piece 12, respectively. Folding hinge 16 engages frame 18 with side groove 22 and peg 20 with hole 24, thereby ensuring that the two parts of device 10 are closed and sealed to prevent contamination of the interior surfaces by dust. The outer surface of closure 14 (not shown in Figures 1 and 2) has flat areas for affixing labels and barcodes to facilitate sample identification. Sampling member 12 and closure member 14 have handles 26 and 28 for opening device 10, respectively.

[0117] A double-coated pressure sensitive medical tape 30 with a protective release liner 32 made of kraft paper, product number #9877 of 3M,...

Embodiment 3

[0123] In order for many samples in Example 2 to be processed together, it is necessary to cut the test piece 40 into a uniform shape that matches the wells of a standard 96-well (8×12) microwell plate. Existing equipment can be used to add reagents to the wells of microplates and to wash the wells, with one requirement being to avoid reagent carryover between steps. The colored solution in the wells can be read directly in the final step of the assay using an existing spectrophotometer. However, during this process, the protruding parts of the test strips outside the wells and the fixtures that hold them prevent the instrument from loading and washing the wells. This results in the need for additional custom equipment for strip assays, and / or more manual steps than conventional microplate assays.

[0124] Batch processing of a large number of samples can be accomplished by cutting a small disc 50 (see, FIG. 4 ) from one side 52 of the sampling device, the disc 50 having an a...

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Abstract

Skin cholesterol is measured by applying an adhesive tape onto a selected area of the skin to adhere the tape to the selected skin area and stripping the tape off the selected skin area to obtain a sample representative of the outer stratum corneum layer of the skin, the sample adhering to the tape so as to have exposed skin constituents. The sample is assayed using a detector reagent that specifically binds to cholesterol and in addition has an indicator component that allows quantitation of the amount of cholesterol present in the exposed skin constituents.

Description

field of invention [0001] The present invention relates to a method for measuring skin cholesterol. More specifically, the present invention relates to a method for directly measuring skin cholesterol in a skin sample obtained by tape stripping, for judging whether an individual suffers from arteriosclerosis, and whether a person has a disease that develops into arteriosclerosis and is associated with and caused by high cholesterol. risk of similar diseases. Background of the invention [0002] Numerous studies have shown that arteriosclerosis and its complications, such as heart attack and shock, are the leading causes of morbidity and death in almost all countries. [0003] Effective prevention of arteriosclerosis requires determining whether a person is at risk for developing arteriosclerosis, enabling appropriate treatment and lifestyle changes. Determining who is at high risk is desirable, but figuring out the best way to determine whether a person is at risk has been...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/60G01N33/92A61B10/00C12Q1/26A61B10/02A61B17/32
CPCG01N33/92A61B17/32053C12Q1/60A61B10/0064A61B10/02A61B2010/0006
Inventor P·豪斯沃德R·扎瓦蒂斯基
Owner 麦拉克林有限公司
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