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Micro fluid phase chromatograph method on micro analysis chip and apparatus of the same

A chromatographic separation and micro-liquid phase technology, applied in separation methods, solid adsorbent liquid separation, measurement devices, etc., can solve the problems of loss of chromatographic column fixed liquid, low separation efficiency, short column life, etc., and achieve low solvent consumption. , The effect of high separation efficiency and large column capacity

Inactive Publication Date: 2007-05-16
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the stationary liquid of the chromatographic column made by the coating method is seriously lost, so the column life is short, and it is gradually replaced by the later bonded stationary phase chromatography.
However, bonded phase chromatography is a monomolecular layer immobilization method, which has the disadvantages of low ratio (stationary phase / mobile phase) and small column capacity.
In order to realize the immobilization of a large amount of fixatives, Ito et al. (Y.Ito; M.A.Weinstein; I.Aoki, Harada; E.Kimura; K.Nunogaki, Nature, 212,985-987.) invented countercurrent chromatography, which is A method based on a gravity field or a high-speed centrifugal force field to stabilize the stationary liquid, but the system is relatively complex, and the separation efficiency is low, requiring expensive high-speed centrifugal equipment and long chromatographic columns (generally, the length of the column is 10-300 meters), so the separation Usually more than 10 hours, even a few days

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  • Micro fluid phase chromatograph method on micro analysis chip and apparatus of the same
  • Micro fluid phase chromatograph method on micro analysis chip and apparatus of the same
  • Micro fluid phase chromatograph method on micro analysis chip and apparatus of the same

Examples

Experimental program
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Effect test

Embodiment 1

[0041]Fig. 1. is the top view of the micro liquid chromatography separation device on a kind of micro analysis chip according to the present invention. Wherein, a series of concave microstructures 5 of the same configuration for trapping the stationary phase 4 are processed on the sidewall of the microchannel 2 of the microchip 1, and the microstructures 5 are rectangular structures with a constriction 9, which is conducive to forming a stable and reproducible Interphase interface 10, processing sampling probe at the inlet 6 of the microchannel. The outlet 7 of the microchannel 2 is connected with a syringe pump through a catheter.

[0042] Before the micro-liquid chromatography separation device is used, the surface of the inner wall of the microstructure 5 in the microchannel 2 is subjected to hydrophobization or hydrophilization treatment. The channel is pre-filled with two mutually miscible liquids (such as lower alcohols, nitriles, ketones, etc.) to remove the gas in the...

Embodiment 2

[0048] Fig. 4. is the top view of the microstructure 5 of the protruding microchannel in the micro liquid chromatography separation device in embodiment 2. Wherein, a series of convex microstructures 5 of the same configuration are processed on the sidewall of the microchannel 2 of the microchip 1 for retaining the stationary liquid phase 4. 3 The flow direction is inclined in the opposite direction. The stationary phase 4 is trapped between the microstructure 5 and the side walls of the microchannel 2 . Arrows indicate the flow direction of the mobile phase. In the experiment, the microchannel 2 is a bent linear structure, the length ranges from 10-50 cm, the width ranges from 50-300 microns, and the depth ranges from 10-50 microns. The inlet 6 of the microchannel 2 is connected with a capillary as a sampling probe. The microstructures 5 have a length of 10-100 microns, a width of 10-50 microns, and a depth of 10-50 microns, and the distance between adjacent microstructure...

Embodiment 3

[0051] 5 is a top view of the microstructure 5 in the middle of the microchannel in the micro-liquid chromatographic separation device in another preferred embodiment 3 of the micro-analysis chip micro-liquid chromatographic separation device according to the present invention. Among them, a series of microstructures 5 for trapping the fixed liquid phase 4 are processed in the center of the microchannel 2 of the microchip 1, and a constriction 9 structure is processed at the opening of the microstructure 5, which is conducive to the formation of a stable and reproducible interphase interface 10 . The microchannel 2 has a length ranging from 1 mm to 10 cm, a linear structure, a width ranging from 10 to 100 microns, and a depth ranging from 50 nanometers to 30 microns. The microstructures 5 have a length of 500 microns to 80 microns, a width of 500 microns to 20 microns, and a depth of 50 nanometers to 30 microns, and the distance between adjacent microstructures 5 is 1 to 50 mi...

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Abstract

This invention relates to one micro liquid phase spectrum isolation method and device of micro analysis chip to realize the isolation operation, which is characterized by the following: the chip micro channel adopts process method on micro array to keep liquid to form spectrum solid phase to fulfill micro shape liquid spectrum isolation operation.

Description

technical field [0001] The field of the invention relates to the field of chromatographic analysis, in particular to a micro-liquid chromatographic separation method and device on a micro-analysis chip. Background technique [0002] Chromatography uses the difference in physical and chemical properties such as the distribution coefficient, adsorption capacity, and affinity of the substance between the mobile phase and the stationary phase. When the two phases move relative to each other, the substance is continuously distributed between the two phases, so as to achieve the purpose of separation. . Chromatography is the core of modern separation and analysis technology. As the most important part of chromatography, chromatographic columns are particularly important. Liquid-liquid partition chromatography based on the principle of liquid-liquid partitioning is currently the most widely used chromatographic technique. The earliest liquid-liquid chromatography column used the m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02B01D15/00
Inventor 方群陈宏祝莹
Owner ZHEJIANG UNIV