Inclined type gravity precipitation live cell trapping device

A technology of gravity sedimentation and interception devices, which is applied to tissue cell/virus culture devices, biochemical instruments, microorganisms, etc., can solve the problems of reactor environment deterioration, long residence time of living cells, cell damage, etc., and reduce deposition and adhesion. Attached, the effect of shortening the average residence time and alleviating the overall activity decline

Inactive Publication Date: 2007-05-23
EAST CHINA UNIV OF SCI & TECH
View PDF2 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The technical problem to be solved by the present invention is to provide a downward inclined gravity sedimentation live cell interception device used in the continuous long-term perfus

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Inclined type gravity precipitation live cell trapping device
  • Inclined type gravity precipitation live cell trapping device

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0048] Example 1

[0049] The settler is combined with a 1L stirring cell culture reactor for perfusion culture of hybridoma cells.

[0050] Cells: The cell line is hybridoma cell HB-58, which is deposited in ATCC (American Tissue Cell Bank), and the deposit number is Hybridoma CELL Line 187.1 (ATCC HB58). Cells are maintained in a 300ml spinner flask, and the spinner flask is placed at 37°C with 5% CO 2 In the incubator, inoculate into the reactor during the logarithmic incubation period.

[0051] The medium is Hybridoma-SFM (1X) (serum-free medium for hybridoma cell culture) manufactured by GIBCO, and the product number is 12045-035.

[0052] The included angle α between the liquid receiving port 3 and the bottom surface of the main body 1 is 45°;

[0053] When in use, the main body 1 is placed obliquely, and the angle β with the horizontal direction is 50°

[0054] Continuous perfusion culture for 60 days. Using glucose-restricted medium (glucose concentr...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a clinism gravitational settling living cell trapping equipment, which contains tilted settling vessel main body (1), feeding throat (2), liquid receiving mouth (3), and refluxing opening (4). It is characterized in that it contains ribs strips (6). Cross section of the said settling vessel main body is oval shape, feeding throat (2) is set on superior extremity of the main body (1), liquid receiving mouth (3) is set at the back of connecting piece of the main body (1), refluxing opening (4) is set at inferior extremity of the main body. The said ribs strips are set in the bottom of the internal surface of the main body. The trapping equipment of this invention is fit for different reactor and has a wide dabbling rate scope. The invention reduces the deposition of cells at bottom of the settling vessel, shortens the mean detention time of cells outside the reactor, and relieves the entire activity descend and environmental aggravation caused by cell deposition.

Description

technical field [0001] The invention relates to an inclined gravity settling living cell intercepting device, in particular to an inclined gravity settling device used for selectively retaining living cells in the continuous perfusion culture process of cells. Background technique [0002] Perfusion culture is an effective way to increase cell density. The advantage of this culture method is that it can continuously add fresh medium without changing the volume of the culture medium, discharge metabolites, make the cells grow rapidly and maintain high activity, so as to achieve high expression and high yield of products. It is a key technical issue to implement continuous perfusion culture to develop a cell interception device and efficiently retain cells. [0003] Among the many cell retention devices, the inclined gravity settling device is considered to be a better cell retention method because of its simple structure, low cost, no clogging, small shearing of cells, and e...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12M3/00C12N5/06
Inventor 谭文松张璐张旭
Owner EAST CHINA UNIV OF SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap