Process for preparing recombinant Sinkiang domestic silkworm antibiotic peptide, Sinkiang domestic silkworm antibiotic peptide therefrom, and use thereof

A silkworm antimicrobial peptide and antimicrobial peptide technology, applied in recombinant DNA technology, fermentation, etc., can solve the problems of low purity, single purification method of recombinant antimicrobial peptide, and high purification cost, and achieve simple operation, tumor growth inhibition, and short time Effect
CN1966709BInactive Publication Date: 2010-06-02XINJIANG UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
XINJIANG UNIVERSITY
Publication Date
2010-06-02
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses the method for purifying and preparing recombinant antibacterial peptide of Xinjiang silkworm, recombinant antibacterial peptide of Xinjiang silkworm, application of recombinant antibacterial peptide of Xinjiang silkworm in bacteriostasis and antitumor. recombinant antibacterial peptide of Xinjiang silkworm is obtained from Xinjiang silkworm through cloning, expressing in eukaryotic expression system. The preparing method is: centrifuging the fermentation broth to remove the precipitant, freeze-drying, separating and purifying through an ion exchange chromatography, a hydrophobic interaction chro-matography, and a gel filtration chromatography to obtain the final product. The recombinant antibacterial peptide of Xinjiang silkworm has high inhibition and killing activity on bacteria and tumor cells, no hemolysis activity, and no toxicity to normal tissue cells, and can be used for preparing drugs for treating infectious diseases caused by pathogenic microorganismand tumors.
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Description

technical field

[0001] The invention belongs to the field of biotechnology. Specifically, it relates to a method for purifying and preparing the recombinant Xinjiang silkworm antibacterial peptide, the recombinant Xinjiang silkworm antibacterial peptide and its application in antibacterial and antitumor aspects. Background technique

[0002] The discovery of penicillin freed people from the fear of diseases caused by pathogenic microorganisms, which led to the emergence of a large number of antibiotics and laid a solid foundation for the protection of human health. However, the abuse of traditional antibiotics and the continuous emergence of drug-resistant strains have seriously affected the clinical treatment of infectious diseases. Therefore, the development of a new generation of antibiotics has become the focus of new drug development in the 21st century. In the past 20 years, antimicrobial peptides from different sources have shown the characteristics of broad-spectru...

Claims

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