Method for constructing allotetraploid of bluntsnout bream

A technique of allotetraploidy and method establishment, applied in the field of fish breeding

Inactive Publication Date: 2007-05-30
李思发
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no efficient method for producing fertile tetraploids of bream

Method used

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  • Method for constructing allotetraploid of bluntsnout bream
  • Method for constructing allotetraploid of bluntsnout bream

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] The establishment of embodiment 1 optimal induction condition

[0060] Orthogonal is diploid bream ♀ × diploid trigonum bream ♂; reciprocal is diploid bream ♀ × diploid bream ♂.

[0061] Set from 1.0τ after fertilization 0 ~2.6τ 0 (That is, when the water temperature is 22°C, from 27min to 60min; at 24°C, from 17min to 50min, τ 0 Proportional to water temperature and time), at different stages of the first mitotic stage of fertilized eggs of bream, heat shock treatment was performed at three different temperatures of 41.0°C, 40.0°C and 39.0°C every 3 minutes, and the duration of treatment 2min or 3min. The fertilized eggs were stuck on the petri dish, and the heat shock was carried out in a constant temperature water bath. Each experimental group was set up with 3-4 parallels, and the embryonic chromosome analysis was carried out in order to establish the appropriate conditions for tetraploid induction.

[0062] The results show that the orthogonal heat shock induct...

Embodiment 2

[0063] The establishment of embodiment 2 head bream allotetraploid

[0064] In the present embodiment, divide into three groups to carry out test altogether:

[0065] Test group 1: adopt the following allotetraploid establishment steps (1)-(7);

[0066] Test group 2: adopt the following allotetraploid establishment steps (1)-(7);

[0067] Experimental group 3: Only interspecies hybridization of bream ♀×triangular bream ♂ was carried out without the heat shock induction step.

[0068] (1) Choose different genetic distance combinations of distant hybridization: Orthogonal bream ♀ × trigonal bream ♂; reciprocal crossing triangular bream ♀ × trigonal bream ♂.

[0069] (2) Select well-mature female and male broodstock, use ovulation-stimulating hormone No. 2 (LHRHa) and human chorionic gonadotropin (HhCG) for mixed induced labor and dry insemination.

[0070] (3) Carry out forward and reverse hybridization.

[0071] (4) Heat shock induction was performed on the hybrid fertilize...

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Abstract

The invention discloses a building method of heterogenous tetraploid of co-head fish, which is characterized by the following: crossing triangle fish and co-head fish; combining different genetic distances; inducing through physical method; utilizing interspecies crossing of fish naturally to form tetraploid; improving the inducing rate of tetraploid after combining physical shock disposal; obtaining triploid fish through crossing tetraploid and diploid.

Description

technical field [0001] The invention relates to the field of fish breeding, and more specifically relates to a method for establishing an allotetraploid fish of the group head bream by utilizing the genome manipulation technology. Background technique [0002] Since the mid-1970s, genome manipulation research has been carried out in more than ten kinds of farmed fish at home and abroad, and some progress has been made, as shown in the following aspects: [0003] 1. Induction of Autotetraploidy [0004] Tetraploids can be obtained by inhibiting the first mitosis of fish fertilized eggs by physical shock (cold shock, heat shock and hydrostatic pressure) induction technology. So far, scholars from all over the world have carried out research on Oria tilapia, rainbow trout, golden perch, tench , catfish, bighead carp, crystal color crucian carp, carp and other fish tetraploid induction. [0005] 2. Induction of Allotetraploidy [0006] Distant hybridization can induce tetra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00A01K67/027A61K38/24A61P15/08A61D19/00
CPCY02A40/81
Inventor 李思发邹曙明蔡完其
Owner 李思发
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