Method for constructing allotetraploid of bluntsnout bream

Abstract

The invention discloses a building method of heterogenous tetraploid of co-head fish, which is characterized by the following: crossing triangle fish and co-head fish; combining different genetic distances; inducing through physical method; utilizing interspecies crossing of fish naturally to form tetraploid; improving the inducing rate of tetraploid after combining physical shock disposal; obtaining triploid fish through crossing tetraploid and diploid.

Description

technical field

[0001] The invention relates to the field of fish breeding, and more specifically relates to a method for establishing an allotetraploid fish of the group head bream by utilizing the genome manipulation technology. Background technique

[0002] Since the mid-1970s, genome manipulation research has been carried out in more than ten kinds of farmed fish at home and abroad, and some progress has been made, as shown in the following aspects:

[0003] 1. Induction of Autotetraploidy

[0004] Tetraploids can be obtained by inhibiting the first mitosis of fish fertilized eggs by physical shock (cold shock, heat shock and hydrostatic pressure) induction technology. So far, scholars from all over the world have carried out research on Oria tilapia, rainbow trout, golden perch, tench , catfish, bighead carp, crystal color crucian carp, carp and other fish tetraploid induction.

[0005] 2. Induction of Allotetraploidy

[0006] Distant hybridization can induce tetra...

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