Antivirus extract of isatis root, its extraction method, use and content assaying method
An isatidis extract, extraction method technology, applied in antiviral agents, chemical instruments and methods, sugar derivatives, etc., can solve the problem of unclear ingredients, difficult to guarantee curative effect and quality, and lack of antiviral active ingredients and material basis of isatis root. To clarify and other issues, to achieve the effect of clear ingredients and positive effects
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Embodiment 1
[0029] Embodiment 1 Radix isatidis antiviral extract is the extraction method of effective part
[0030] Dry slices of Radix isatidis 2kg, extract with alcohol aqueous solution for 2h and 1.5h respectively, filter, combine the two extracts and concentrate to 4kg, and filter. While stirring, add 95% ethanol to the extract until the ethanol content reaches 60-80%. Let stand overnight, filter with suction, recover the solvent from the filtrate, and concentrate to 2kg. The Radix Radix isatidis extract was repeatedly absorbed by the macroporous resin, eluted with different concentrations of ethanol, and the ethanol eluate was collected, concentrated and dried to obtain 17 g of refined effective fractions.
Embodiment 2
[0031] Example 2 Determination of Content of Larix Resinol-4,4'-Di-O-β-D-Glucopyranoside in Radix Radix Radix Larici
[0032] 2.1 Instruments and reagents
[0033] Shimadzu CBM-10A high performance liquid chromatography system; SPD-10A ultraviolet detector; CLASS SAILNET Driver workstation.
[0034] The reference substance of larch resinol-4,4'-di-O-β-D-glucopyranoside was isolated from Radix Radix Radix and identified as larchoresin-4,4' by UV, IR, NMR, MS, etc. - Di-O-β-D-diglucoside (clemastanin B). It is a single component detected by HPLC and TLC, and the purity detected by HPLC peak area normalization method is 99.2%.
[0035] Radix isatis medicinal material was purchased from major producing areas such as Jiangsu, Anhui, and Hebei, and was identified as the dried root of Isatis indigotica Fort. Reagents such as methanol and acetonitrile were chromatographically pure.
[0036] 2.2 Methods and results
[0037] 2.2.1 Determination method
[0038] Preparation of refer...
Embodiment 3
[0055] Example 3 The preparation method of standard substance larch resinol-4,4'-two-O-β-D-glucopyranoside
[0056] 3.1 Preparation method
[0057] 1 kg of Radix isatidis powder was refluxed twice with 80% methanol aqueous solution, and the extract was concentrated under reduced pressure to obtain 65 g of extract. The extract was dissolved in 1000ml of water under heating in a water bath, passed through the macroporous resin, and the ethanol eluted part was collected, concentrated under reduced pressure, and dried to obtain 12g. The sample was separated by silica gel column chromatography, and the pure product of larch resinol-4,4'-di-O-β-D-glucopyranoside was used as the reference substance for TLC tracking detection, and the fractions with the same spots as the reference substance were merged , after recovering the solvent, recrystallize twice through water and methanol respectively to obtain 120 mg of standard larch resinol-4,4'-di-O-β-D-glucopyranoside.
[0058] 3.2 Conf...
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