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Compositions and methods for stabilizing lipid based adjuvant formulations using glycolipids

A stable, glycolipid-based technology, applied in liposomes with sterically stabilized cations, for immunization in the field of vaccines with adjuvants in aqueous media, which can solve problems such as physical instability

Active Publication Date: 2011-10-12
STATENS SERUM INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0022] Since quaternary ammonium compounds, especially DDA, are very promising candidates for effective vaccine adjuvants, but have major disadvantages of being physically unstable in aqueous solutions, forming aggregates and precipitates during storage, there is a need to stabilize the formed small vesicle

Method used

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  • Compositions and methods for stabilizing lipid based adjuvant formulations using glycolipids
  • Compositions and methods for stabilizing lipid based adjuvant formulations using glycolipids
  • Compositions and methods for stabilizing lipid based adjuvant formulations using glycolipids

Examples

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Embodiment Construction

[0024] The invention discloses a novel method for stabilizing cationic liposomes with glycolipids in aqueous preparations. For example, cationic liposomes made from amphiphilic quaternary ammonium compounds are stabilized by the incorporation of glycolipids in the liposome membrane.

[0025] A preferred embodiment of the present invention is when the quaternary ammonium compound is bromate, hydrochloride, sulfate, phosphoric acid of dimethyl dioctadecyl ammonium (DDA) or dimethyl dioctadecyl ammonium (DODA). salt or acetate compounds.

[0026] Other preferred quaternary ammonium compounds are 1,2-dioleoyl-3-trimethylammonium propane (DOTAP), 1,2-dimyristoyl-3-trimethylammonium propane, 1,2-dipalmitoyl -3-trimethylammonium propane, 1.2-distearoyl-3-trimethylammonium propane, and dioleoyl-3-dimethylammonium propane (DODAP) and N-[1-(2,3- Dioleoyloxy)propyl]-N,N,N-trimethylammonium (DOTMA).

[0027] The glycolipid used to stabilize liposomes is preferably α,α'-trehalose 6,6'di...

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Abstract

The present invention relates to liposome formulations that are physically stable. In particular the present invention relates to steric stabilization of cationic liposomes by incorporating glycolipids into the liposomes. The stabilized liposomes can be used either as an adjuvant for antigenic components or as a drug delivery system. In particular the invention relates to vaccines with adjuvants in aqueous media for immunization, where the final product is stable.

Description

technical field [0001] The present invention relates to physically stable liposomal formulations. In particular, the present invention relates to sterically stabilized cationic liposomes by a unique membrane approach in which glycolipids are incorporated into the liposomes. Stabilized liposomes can act as adjuvants for antigenic components or as drug delivery systems. In particular, the invention relates to vaccines with adjuvants in aqueous media for immunization, when the final product is stable. Background technique [0002] The first vaccines used in humans to confer immunity to infectious diseases consisted of live, attenuated pathogens. The attenuated form either occurs naturally in close association with the organism or is acquired by serial passage in culture. An example is tuberculosis, which is combated by a weakened but active strain of Mycobacterium bovis (BCG vaccine). However, the effectiveness of this approach does not provide satisfactory resistance to tu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/26A61K47/18A61K9/127A61K39/015A61K39/04A61K39/118A61P31/00A61P31/06A61P33/06A61P37/04
CPCA61K9/1272A61K39/04A61K2039/55555A61P31/00A61P31/06A61P33/06A61P37/04Y02A50/30A61K9/127A61K39/118A61K39/015
Inventor 贾斯博·大卫德森艾达·罗森克兰达斯彼得·安德森
Owner STATENS SERUM INST