Culture process of yard grass Bipolaris sacchari and its use

A technology of umbilical sp. and its cultivation method, which is applied in the field of cultivation of umbilical sp. cricket, and can solve the problems of single species aging and high cost.

Inactive Publication Date: 2007-06-27
CHINA NAT RICE RES INST
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Problems solved by technology

Domestic control of rice sheath blight mainly uses Jinggangmycin. Although Jinggangmycin is a low-toxic antibiotic biopesticide, Jinggangmycin has bee...

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  • Culture process of yard grass Bipolaris sacchari and its use

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[0021] The present invention will be further described in detail through the following examples and test examples.

[0022] Take the leaves of susceptible barnyardgrass, wash them with tap water, cut the leaves into appropriate sizes, disinfect them with 70% alcohol, place them on PDA medium, and keep them moist at room temperature 28°C. After 4 days, Helminoptera umbilicaliformis was observed under the dissecting microscope The conidia of the genus were picked out with a flame-sterilized dissecting needle, spread on the PDA medium and cultivated for 7 days, and the agar block containing only one spore was taken, transferred to the PDA slant to cultivate a monospore system, and then transferred to After receiving the barnyardgrass seeds and culturing them for 10 days, they were divided into sterile 1.5mL Eppendorf centrifuge tubes, and the strains were preserved at 4°C.

[0023] Take a barnyardgrass seed with hyphae and spores and inoculate it on a fresh PDA plate. After culti...

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Abstract

The present invention is culture process and use of yard grass Bipolaris sacchari, and belongs to the field of plant pathogenic fungus technology. The culture process includes the following steps: sterilizing collected infected cockspur grass sample, culturing in PDA culture medium at room temperature for 3-4 days until conidiophore of Bipolaris sacchari is observed under anatomical lens, picking out with flame sterilized dissecting needle and setting on PDA culture medium, transferring for PDA slant cultivation to form single spore line, culturing in cockspur grass seed for 10 days, packing in abacterial Eppendorf centrifuge tube and preserving at 4 deg.c; taking one cockspur grass seed with hypha and spore and inoculating to fresh PDA plate, dark culturing at 28 deg.c for 7 days, inoculating 7 mm diameter fungus block onto PDB liquid culture medium and static culturing at 28 deg.c for 14 days. Yard grass Bipolaris sacchari and its fermented liquid may be used in preparing biological herbicide and fungistat.

Description

technical field [0001] The invention belongs to the technical field of plant pathogenic fungi, and in particular relates to a cultivation method and application of Umbilocorcus cricket. Background technique [0002] Barnyardgrass is one of the most difficult weeds to control in the world, and currently it mainly relies on chemical herbicides to control it. However, the long-term use of chemical herbicides has made the resistance (resistance) of barnyardgrass and the problem of environmental pollution more and more serious. The development of safe and efficient biological herbicides meets the requirements of sustainable agricultural development. Although biological herbicides cannot completely replace chemical herbicides, they can effectively reduce the dosage of chemical herbicides, thereby reducing environmental pollution. [0003] Rice sheath blight is a global rice disease caused by the soil fungus Rhizoctonia solani kuhn, which seriously restricts rice production. Rice...

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P3/00A01P13/00
Inventor 张建萍余柳青
Owner CHINA NAT RICE RES INST
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