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Truffle polyose preparing process

A technology of bacterial polysaccharides and maltose, applied in the field of submerged liquid fermentation to produce truffle polysaccharides, can solve the problems of insufficient yield of wild truffles, difficult quality assurance, heavy workload, etc., and achieve continuous high-efficiency production, low cost, and prevent pesticides residual effect

Inactive Publication Date: 2007-06-27
HUBEI UNIV OF TECH
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AI Technical Summary

Problems solved by technology

[0006] Although semi-artificial simulated cultivation can solve the problem of insufficient yield of wild truffles to a certain extent, it generally takes 7-9 years from inoculation to harvest, which is a long cycle and a large investment
During the cultivation process, special management personnel are required to fertilize, irrigate, and protect it from wild animals. The workload is heavy and the effect is slow. At the same time, it is greatly affected by factors such as the climate in the natural environment, and the quality is difficult to guarantee.
[0007] Therefore, if the fruiting bodies of truffles are used as raw materials for the industrial production of truffle polysaccharides, neither wild nor semi-artificial simulated cultivation can solve the problems of high price and limited output.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The strain used: Chinese truffle (Tuber sinense Tao et Liu).

[0023] A slant strain culture: the formula of the slant strain culture medium adopted in the present embodiment is: maltose 50 grams / liter, magnesium sulfate 5 grams / liter, potassium dihydrogen phosphate 5 grams / liter, agar 15 grams / liter, potato The extract is 1.0 liters, the pH value is 5 or 6 or 7 or 8. Sterilization conditions are: 121°C, 20 minutes. The culture temperature is 15 or 18 or 22 or 27 or 30 or 34 or 37 or 40° C., and the culture time is 2 or 4 or 8 or 10 or 12 or 15 or 17 or 20 days.

[0024]B first-level liquid seed culture: the formulas of the first-level liquid seed and the second-level liquid seed medium in this embodiment are: maltose 70 grams / liter, peptone 25 grams / liter, magnesium sulfate 5 grams / liter, potassium dihydrogen phosphate 5 g / L, pH value is the same as step A. The first-level liquid seed culture can be carried out by transferring the slant bacteria into the shake flask...

Embodiment 2-9

[0029] The concentrations of maltose in the slant culture medium are 30 g / L, 50 g / L, 60 g / L, 70 g / L, 90 g / L, 110 g / L, 130 g / L, 150 g / L , other culture conditions are identical with embodiment 1. Analysis and test results showed that the yields of truffle exopolysaccharides were 1.23 g / L, 1.60 g / L, 1.95 g / L, 1.78 g / L, 1.65 g / L, 1.56 g / L, 1.06 g / L, and 0.81 g / Lift.

Embodiment 10-17

[0031] The maltose in the slant culture medium is 60 grams / liter, and the concentration of magnesium sulfate in the slant culture medium is 0.1 gram / liter, 0.5 gram / liter, 1 gram / liter, 2 gram / liter, 5 gram / liter, 7 grams / liter, 9 grams / liter, 10 grams / liter, other culture conditions are identical with embodiment 1. Analysis and test results showed that the yields of truffle exopolysaccharides were 0.21 g / L, 0.26 g / L, 0.56 g / L, 1.54 g / L, 2.01 g / L, 1.85 g / L, 1.06 g / L, and 0.93 g / Lift.

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Abstract

The truffle polyose preparing process includes the following steps: inoculating Chinese truffle seed to fresh slant culture medium for slant culturing, inoculating the slant cultured truffle seed to the first liquid seed culture medium inside shake flask for the first liquid seed culturing, transferring the first liquid seed to the second seed culture medium inside shake flask for the expanded second liquid seed culturing, transferring the second liquid seed to a shake flask for deep fermentation, and determining truffle amylovorin. The present invention has amylovorin yield in the fermented liquid up to 2.30 g / l, and the prepared truffle amylovorin possesses tumor inhibiting activity. The present invention has simple operation and low cost, and suitable for industrial production.

Description

technical field [0001] The invention belongs to the field of bioengineering and technology, and specifically relates to a method for producing truffle polysaccharide by liquid submerged fermentation, which is suitable for industrialized large-scale production of developing truffle polysaccharide into anticancer and immunity-enhancing drugs. Background technique [0002] Truffles belong to Ascomycotina, Tuberales, Tuberaceae, and Tuber in taxonomy. Common truffles include Tuber melanosporum Vittad, Tuber indicum Cooke, and Chinese truffles (Tuber sinense Tao et Liu). At present, the price per kilogram of fresh black spore truffle in the international market is as high as more than 1,000 US dollars. Its expensive price and black, warty appearance endow it with the reputation of "black diamond" in the forest. Truffle polysaccharide is the main biologically active substance in truffles. Truffle polysaccharide is a kind of glycoprotein with good w...

Claims

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Application Information

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IPC IPC(8): C12P19/04C12R1/645
Inventor 汤亚杰李冬生孔国平
Owner HUBEI UNIV OF TECH
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