Pure breed fermentation process for producing tasteless preserved soybean
A pure-bred fermentation and light tempeh technology, applied in the field of fermentation engineering, can solve the problems of carcinogenicity, fermentation failure, poor fermentation production stability, etc., and achieve the effects of improving stability, improving safety, and ensuring consistency
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Embodiment 1
[0023] (1) Soaking and cooking of soybeans
[0024] Take 100 grams each of mulberry leaves and Artemisia annua, add 2500 grams of water to decoct, filter, mix the decoction into 1000 grams of washed soybeans, and cook under high pressure at 0.1 MPa for 40 minutes after the decoction is exhausted.
[0025] (2) Preparation of seed solution
[0026] Using KMB liquid medium, insert the strain CGMCC No.1863 under aseptic conditions, and cultivate it on a shaker at 150rpm for 18 hours, count the colonies on the nutrient agar plate, and dilute it with sterile physiological saline to contain 10 live bacteria. 7 per milliliter of seed solution.
[0027] (3) Inoculation of koji making and post-fermentation
[0028] After inoculating with the weight-volume ratio (w / v) of boiled soybeans and seed liquid being 100:1, place it at 37°C for 48 hours, wash the koji, place it in a closed container at 45°C for 7 days, take it out and dry it.
[0029] The surface of this product is black, wrin...
Embodiment 2
[0031] (1) Soaking and cooking of soybeans
[0032] Take 100 grams each of mulberry leaves and Artemisia annua, add 2500 grams of water to decoct, filter, mix the decoction into 1000 grams of washed soybeans, and cook under normal pressure for 3 hours after exhaustion.
[0033] (2) Preparation of seed solution
[0034] Using KMB liquid medium, insert the strain CGMCC No.1863 under aseptic conditions, culture it on a shaker at 150rpm for 18 hours, count the colonies on the nutrient agar plate, and dilute it with sterile normal saline to contain 10 live bacteria. 7 per milliliter of seed solution.
[0035] (3) Inoculation of koji making and post-fermentation
[0036] After inoculating the weight-volume ratio (w / v) of boiled soybeans to seed liquid as 100:3, place it at 30°C for 60 hours, wash the koji, then place it in a closed container at 50°C for 10 days, take it out and dry it.
[0037] The surface of this product is black, wrinkled and uneven, the cross-section is brown,...
Embodiment 3
[0039] (1) Soaking and cooking of soybeans
[0040] Take 100 grams each of mulberry leaves and Artemisia annua, add 2500 grams of water to decoct, filter, mix the decoction into 1000 grams of washed soybeans, and cook under high pressure at 0.05 MPa for 60 minutes after exhaustion.
[0041] (2) Preparation of seed solution
[0042] Using KMB liquid medium, insert the strain CGMCC No.1863 under aseptic conditions, culture it on a shaker at 150rpm for 18 hours, count the colonies on the nutrient agar plate, and dilute it with sterile normal saline to contain 10 live bacteria. 7 per milliliter of seed solution.
[0043] (3) Inoculation of koji making and post-fermentation
[0044] After inoculation according to the amount of steamed soybean: seed liquid = 100: 2 (w / v), place it at 33°C for 48 hours, wash the koji, place it in a closed container at 55°C for 8 days, take it out and dry it.
[0045] The surface of this product is black, wrinkled and uneven, the cross-section is b...
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