Non-Absorbent Articles Including Aromatic Compositions As Inhibitors Of Exoprotein Production
a non-absorbent, non-absorbent technology, applied in the direction of bandages, biocide, animal husbandry, etc., can solve the problems of difficult to eradicate i>s. aureus /i>bacterium without harming members, and the use of antibiotics that kill i>s. aureus /i>is not an option in products, so as to inhibit the production of exoproteins
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example 1
[0072] In this Example, the effect of various test compounds on the growth of S. aureus and the production of TSST-1 was determined. The test compound, in the desired concentration (expressed in percent of active compound) was placed in 10 mL of a growth medium in a sterile, 50 mL conical polypropylene tube (Sarstedt, Inc. Newton, N.C.).
[0073] The growth medium was prepared by dissolving 37 grams of brain heart infusion broth (BHI) (Difco Laboratories, Cockeysville, Md.) in 880 mL of distilled water and sterilizing the broth according to the manufacturer's instructions. The BHI was supplemented with fetal bovine serum (FBS) (100 mL) (Sigma Chemical Company, St. Louis, Mo.). Hexahydrate of magnesium chloride (0.021 M, 10 mL) (Sigma Chemical Company, St. Louis, Mo.) was added to the BHI-FBS mixture. Finally, L-glutamine (0.027 M, 10 mL) (Sigma Chemical Company, St. Louis, Mo.) was added to the mixture.
[0074] Compounds to be tested included phenylethyl alcohol, benzyl alcohol, and 2-...
example 2
[0081] In this Example, the effect of various test compounds on the growth of S. aureus and the production of TSST-1 was determined. The effect of the test compounds tested in Example 2 was determined by placing the desired concentration, expressed in percent of the active compound, in 10 mL of a growth medium as described in Example 1. The test compounds were then tested and evaluated as in Example 1.
[0082] In accordance with the present invention, Table 2 shows that S. aureus (MN8), when compared to the control, produced significantly less TSST-1 in the presence of the aromatic compounds. The aromatic compounds reduced the amount of exotoxin production ranging from about 82% to 97%. However, although the amount of toxin produced was significantly reduced, there was minimal, if any, effect on the growth of S. aureus cells.
TABLE 2ELISA:% TestTSST-1ReductionCom-Opticalng / ODofCompoundpoundDensityCFU / mLunitToxin %Growth MediumZero0.607>1.6E+09 2424N / AMethanol400 μL0.5982.6E+092690N...
example 3
[0083] In this Example, the effect of various test compounds on the growth of S. aureus and the production of TSST-1 was determined. The effect of the test compounds tested in Example 3 was determined by placing the desired concentration, expressed in percent of the active compound, in 10 mL of a growth medium as described in Example 1. The test compounds were then tested and evaluated as in Example 1.
[0084] In accordance with the present invention, Table 3 shows that S. aureus (MN8), when compared to the control, produced significantly less TSST-1 in the presence of the aromatic compounds. The aromatic compounds reduced the amount of exotoxin production ranging from about 69% to 98%. However, although the amount of toxin produced was significantly reduced, there was minimal, if any, effect on the growth of S. aureus cells.
TABLE 3ELISA:TSST-1% TestOpticalng / ODReductionCompoundCompoundDensityCFU / mLunitof Toxin %GrowthZero0.6273.9E+091931N / AMediumMethanol100 uL0.5885.2E+092041N / APh...
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