Enhanced Floral Sink Strength and Increased Stability of Seed Set in Plants
a technology of plant seed set and sink strength, applied in the field of plant molecular biology, can solve the problems of less effective competition and decrease in the genetic yield potential of plants, and achieve the effect of enhancing the sink strength or ability of a particular plant tissu
Inactive Publication Date: 2007-07-05
TOMES DWIGHT T +2
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Benefits of technology
This approach enhances yield potential and stress resistance by ensuring adequate carbohydrate supply to sink tissues, maintaining growth and development, particularly during stress conditions, thereby buffering vulnerable periods like anthesis.
Problems solved by technology
Kernel abortion increases when unfavorable environments occur around flowering, thereby decreasing genetic yield potential in plants.
Developing female florets are weak sinks (relative to male florets) and compete less effectively when assimilate is limiting.
Method used
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Embodiment Construction
[0043] The present invention is based on isolation and characterization of genes affecting the flux and availability of the photosynthate products which are sink strength enhancers such as starch and sucrose in plants. Any nucleotide sequence encoding an enzyme or metabolite in the starch or sucrose metabolic or anabolic pathways may be used in accordance with the present invention. Nucleotide sequences encoding these enzymes or substrates are easily ascertainable to those of skill in the art through Genbank or the references disclosed herein. For example, in starch storage organs of some plants, it is believed that sucrose is broken down in the cytoplasm into fructose plus UDP-glucose by sucrose synthase. UDP-glucose is then converted into glucose-1-phosphate by UDP-glucose pyrophosphorylase. Once in the amyloplasts of storage organs, glucose-1-phosphate is converted to ADP-glucose by (AGPase). ADP-glucose is then utilized by starch synthase to add a glucopyranosyl residue to the n...
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Abstract
The invention discloses a transgenic method for enhancing sink strength in female reproductive organs. It involves the temporal and spatial expression of genes affecting the starch and sucrose metabolic pathways in a plant organ or plant tissue. The invention also includes expression constructs, and methods for the production of crop plants with heritable phenotypes which are useful in breeding programs designed to increase yield potential over a range of environmental conditions.
Description
CROSS-REFERENCE TO RELATED APPLICATION [0001] This application is a Continuation of U.S. application Ser. No. 10 / 462,465 filed Jun. 16, 2003, which is a Continuation of U.S. application Ser. No. 09 / 599,353 filed Jun. 20, 2000, now abandoned, which is a nonprovisional application claiming the benefit of U.S. Provisional Application Ser. No. 60 / 140,314, filed Jun. 21, 1999; which applications are hereby incorporated by reference in their entirety.FIELD OF THE INVENTION [0002] This invention relates generally to the field of plant molecular biology. More specifically, this invention relates to methods and reagents for the temporal and spatial expression of genes that enhance sink strength in plants, especially transgenic plants, to increase yield and health of plants in general as well as in periods of stress. BACKGROUND OF THE INVENTION [0003] The yield of a crop or ornamental plant ultimately depends on the energy the plant gains through the fixing of carbon dioxide (CO2) into carboh...
Claims
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IPC IPC(8): A01H5/00C12N15/82C12N5/04C12N15/54
CPCC12N15/8261C12N15/8245Y02A40/146
Inventor TOMES, DWIGHT T.ZINSELMEIER, CHRISTOPHERHABBEN, JEFFREY E.
Owner TOMES DWIGHT T