Method and apparatus for precise selection and extraction of a focused component in isoelectric focusing performed in micro-channels

a microchannel and focused component technology, applied in the field of methods and equipment, can solve the problems of unavoidable component zone broadening, cross-contamination of closely migrating peaks, and inability to achieve more conventional separatory methods
US20080035484A1Inactive Publication Date: 2008-02-14PROTEINSIMPLE
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Patent Information

Authority / Receiving Office
US · United States
Patent Type
Applications(United States)
Current Assignee / Owner
PROTEINSIMPLE
Publication Date
2008-02-14
Estimated Expiration
Not applicable · inactive patent

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Abstract

An apparatus and method are disclosed for the precise selection and extraction of a selected analyte in a focused zone produced by isoelectric focusing performed in micro-channels. A cross-channel microfluidic device comprises a sample mixture introduction and separation channel and an extraction channel, which are in fluid communication with each other at a point of intersection. Means are provided for selectively moving the pattern of separated zones following cIEF to the intersection point, and means are provided for applying an extraction pressure to direct a single zone containing a selected analyte into and then out of the extraction channel for collection.
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Description

CROSS REFERENCE TO RELATED APPLICATIONS

[0001] This application claims the benefit under 35 U.S.C. § 119(e) of U.S. Provisional Patent Application No. 60 / 819,390, filed on Jul. 10, 2006, the disclosure of which is incorporated by reference herein.STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH OR DEVELOPMENT

[0002] N / A FIELD OF THE INVENTION

[0003] This invention relates to the separation and precise selection and extraction of components of an analyte mixture by means of liquid-phase capillary or micro-channel electrophoresis. The principal application of the invention is to facilitate the identification and characterization of the extracted component(s) through the subsequent use of micro-analytical techniques such as mass spectrometry. BACKGROUND OF THE INVENTION

[0004] Capillary electrophoresis (CE) has been established as an important separation technique in bioanalytical chemistry. Separation and detection of very small amounts of biological samples, about pL-nL volumes, can ...

Claims

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