Prodrugs of vancomycin with hydrolysis resistant polymer linkages

a vancomycin and hydrolysis resistant technology, applied in the field of prodrugs, can solve the problems of not providing sufficient active compounds, and achieve the effects of prolonging the circulating life, reducing dose frequency and its related medical costs, and maintaining serum levels

Inactive Publication Date: 2009-09-03
ENZON PHARM INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0032]One advantage of the compounds of the invention is that vancomycin conjugate has an extended circulating life. There is also provided a means for controlling the rate of hydrolysis of the derivative. Thus, the artisan has the ability to include varied substituents that allow for modulation of the rate of hydrolysis of the prodrug to optimize the pK profile, reduce dose frequency and its related medical costs. The modifications described herein also allow one to maintain serum levels and prevent bacterial resistance of vancomycin from developing.
[0033]The conjugates made by the methods of the invention also provide an economic advantage in the manufacturing process using high molecular weight polymers.

Problems solved by technology

In the past, it was thought that using permanent bonds, i.e. those substantially resistant to hydrolysis, to attach the vancomycin to a polymer such as PEG would not provide sufficiently active compounds.

Method used

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  • Prodrugs of vancomycin with hydrolysis resistant polymer linkages
  • Prodrugs of vancomycin with hydrolysis resistant polymer linkages
  • Prodrugs of vancomycin with hydrolysis resistant polymer linkages

Examples

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example 1

[0148]Compound 4. A solution of 2 (mw 40 kDa, 23 g, 0.575 mmol) and disuccinimidyl carbonate (DSC, 2.36 g, 9.2 mmol) in methylene chloride (DCM, 230 mL) and dimethylformamide (DMF, 23 mL) was cooled to 0° C., followed by the addition of pyridine (0.75 mL, 9.2 mmol). This mixture was allowed to warm to room temperature overnight, followed by filtration through Celite® and partial removal of the solvent from the filtrate by rotary evaporation under reduced pressure. The crude product was precipitated with ether and collected by filtration, and crystallized from 20% DMF / isopropanol (IPA) to yield 4 (20.1 g, 0.496 mmol, 86%). 13C NMR (67.8 MHz, C5D5N) δ 168.2, 151.1, 70.7-69.6 (PEG), 68.0, 45.2, 25.2.

example 2

[0149]Compound 5. To a solution of 1 (0.550 g, 0.37 mmol) and triethylamine (TEA, 2.06 mL, 14.8 mmol) in DMF (50 mL) was added 4 (3 g, 0.074 mmol) and 5.5 g molecular sieves (4 Å) and the mixture stirred at 30° C. for 5 hrs. The reaction mixture was filtered through celite, the PEG conjugate precipitated with ether, filtered, and crystallized from DMF / ethanol (50:50) three times to give 5 (2.0 g, 0.0436 mmol, 59%).

example 3

[0150]Compound 6. A solution of 2 (10 g, 0.025 mmol) in toluene (150 mL) was azeotroped for 2 hrs with the removal of 50 mL of distillate. The reaction mixture was then cooled to 30° C., followed by the addition of a 1.0 M solution potassium t-butoxide in t-butanol (1.7 ml, 1.7 mmol). The resulting mixture was stirred for 1 hr at 45° C., cooled to 30° C., followed by the addition of t-butyl bromoacetate (0.4 g, 2.0 mmol). The resulting mixture was refluxed for 18 hrs, followed by filtration through Celite and partial removal of the solvent from the filtrate by rotary evaporation under reduced pressure. The crude product was precipitated with ether, collected by filtration, and crystallized from IPA to yield 6 (8.1 g, 0.20 mmol, 80%). 13C NMR (67.8 MHz, C5D5N) δ 169.1, 81.1, 72.2-69.6 (PEG), 68.7, 45.2, 27.9.

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Abstract

Vancomycin-polymer conjugates are disclosed. In preferred aspects, polymer residues which are hydrolysis resistant in vitro, are selectively attached to the sugar amino and / or N-methyl amino groups of vancomycin and related compounds. Vancomycin-polymer conjugates made by the methods and methods of treatment using the conjugates are also disclosed.

Description

CROSS REFERENCE TO RELATED APPLICATION[0001]This patent application is a continuation of U.S. patent application Ser. No. 10 / 705,740 filed on Nov. 11, 2003, which claims the benefit of priority from U.S. provisional patent application Nos. 60 / 425,890 and 60 / 425,892, each filed Nov. 12, 2002. The contents of each application are incorporated herein by reference.FIELD OF THE INVENTION[0002]The present invention relates to polymeric derivatives of vancomycin. More particularly, the invention relates to select vancomycin derivatives in which the sugar amino group and / or the N-methyl amino group has been modified with a substantially non-antigenic polymer through a hydrolysis resistant linker.BACKGROUND OF THE INVENTION[0003]Vancomycin is an antibiotic which was initially discovered in the 1950's, see U.S. Pat. No. 3,067,099. It is usually reserved for use in the treatment of severe gram positive infections such as those caused by Staphylococcus aureus and when traditional antibiotics ha...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K38/14C07K9/00A61P31/04C08H1/00C12Q
CPCA61K47/48253A61K47/48215A61K47/60A61K47/641A61P31/04
Inventor GREENWALD, RICHARD B.ZHAO, HONG
Owner ENZON PHARM INC
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