Biological regenerate
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example 1
Stem Cell Isolation, Preparation for Transplantation, and Analyses
[0055]Approximately 100 to 200 ml of subcutaneous abdominal fat was harvested in an operation and transported to REGEA Institute for Regenerative Medicine for stem cell isolation and expansion. Additionally, 50 to 60 ml of autologous serum was also obtained for the expansion of the autologous stem cells.
[0056]ASCs were isolated and expanded in vitro in GMP-class clean rooms according to Standard Operation Procedures at REGEA Institute for Regenerative Medicine. Isolation was carried out using mechanical and enzymatic isolation as described previously (Gimble et al., Cytotherapy 2003; 5:362-9; 28. Niemelä et al., J Craniofac Surg 2007; 18:325-35). Briefly, the adipose tissue was minced into small fragments and digested with collagenase type I (Invitrogen, Paisley, Scotland, UK) to separate the adipose derived stem cells from the surrounding tissue. The isolated cells were expanded 14 days in basal media (BM) containing...
example 2
Maxillary Reconstruction of Patient 1
[0065]The patient was 65-year-old male, who underwent a hemimaxillectomy 28 months earlier due to a large recurrent keratocyst. The orbital floor had been reconstructed with a calvarial bone graft covered with temporal muscle. During the follow-up, the patient had a removable obturator prosthesis in the area and no signs of recurrence were noted.
[0066]In the first operation approximately 200 ml of subcutaneous abdominal fat was harvested, and 60 ml of autologous serum was collected. The fat and serum were transported to REGEA for stem cell isolation and expansion as described in Example 1.
[0067]The second operation was performed in general anesthesia 16 days after the first operation. In this operation, a preformed titanium cage filled with ASCs and beta-TCP was inserted carefully through vertical incision in a pouch prepared in the patient's left rectus abdominis muscle. Care was taken not to disturb the vascular supply of the muscle. The incisi...
example 3
Maxillary Reconstruction of Patient 2
[0071]The patient was a 59-year old male, who had undergone a complete tooth extraction of the maxilla 30 years ago. The patient was suffering from inadequately fitting dentures.
[0072]In the first operation, approximately 200 ml of subcutaneous abdominal fat was harvested and 60 ml of autologous serum was collected. The fat and serum were transported to REGEA for stem cell isolation and expansion as described in Example 1.
[0073]In the second operation, the stem cells and beta-TCP were put in the titanium cage which was shaped using the stereolithography model. The left latissimus dorsi muscle was exposed through a lateral thoracic incision. The muscle was separated laterally and its vascular pedicle (thoracodorsal artery and venae comitantes) was identified. Three to four cm distally to the area were the pedicle runs into to latissimus dorsi muscle the muscle was open laterally and the pouch for the cage with ASCs and beta-TCP was formed. The cag...
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