Biomarkers for subtypes of cervical cancer
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Gene Dosage, Expression, and Ontology Analysis Identifies Driver Genes in the Carcinogenesis and Chemoradioresistance of Cervical Cancer
[0151]Integrative analysis of gene dosage, expression, and ontology (GO) data was performed to discover driver genes in the carcinogenesis and chemoradioresistance of cervical cancers. Gene dosage and expression profiles of 102 locally advanced cervical cancers were generated by microarray techniques. Fifty-two of these patients were also analyzed with the Illumina expression method to confirm the gene expression results. An independent cohort of 41 patients was used for validation of gene expressions associated with clinical outcome. Statistical analysis identified 29 recurrent gains and losses and 3 losses (on 3p, 13q, 21q) associated with poor outcome after chemoradiotherapy. The intratumor heterogeneity, assessed from the gene dosage profiles, was low for these alterations, showing that they had emerged prior to many other alterations and probab...
example 2
Integrated Genomic and Transcriptional Profiling of Cervical Cancers Reveals Candidate Biomarkers of Chemoradioresistant Disease
[0188]Purpose. Gene dosage alterations like gains and losses influence gene expressions and are motive forces for tumor development and progression. The purpose of this work was to identify gene dosage alterations and the affected transcripts in locally advanced cervical cancers, and explore their role in development of chemoradioresistant disease.
[0189]Materials and Methods. A total of 102 patients with locally advanced squamous cell carcinoma of the uterine cervix were included. All patients received radiotherapy with or without cisplatin. Tumor biopsies were taken before the start of treatment and used for the molecular profiling. Gene dosage and expression profiling was performed by array comparative genomic hybridization (aCGH) and cDNA microarray analysis, respectively. Pairwise data were available for 95 patients. The GISTIC and LASSO methods, suitab...
example 3
RYBP Protein Expression and Cellular Localization
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[0192]Immunohistochemistry. Pre-treatment tumor specimens were available in sufficient amounts for immunohistochemical study in 150 patients. Formalin-fixed, paraffin-embedded tissue sections from the patients and three cell lines (HeLa, SiHa, CaSki) were immunostained with RYBP (LifeSpan BioSciences, Seattle, Wash.) using the Dako EnVision™ FLEX+ detection system (Dako Corp., Denmark) manually. Heat induced epitope retrieval was performed with a PT Link using Envsion™ FLEX Target Retrieval Solution at high pH (Tris / EDTA buffer pH 9). Incubation time for the primary antibody was 45 minutes at room temperature. EnVision™ FLEX+ Rabbit LINKER was used for signal amplification of primary antibody and the reaction was visualized by EnVision™ FLEX DAB+ Chromogen. Placenta was used as positive control. As negative control, the primary antibody was substituted with normal rabbit IgG of the same concentration as the primar...
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