Genetic analysis loc with hybridization array with calibration chamber containing chamber with a blocked inlet spotted with reporter

a gene analysis and hybridization array technology, applied in the field of diagnostic devices, can solve the problems of slow growth of this type of testing in the clinical laboratory, reduced sensitivity, and high degree of non-specific binding, and achieve the effect of improving the reliability, sensitivity, and dynamic range of analytical outcomes

Inactive Publication Date: 2011-12-22
GENEASYS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Benefits of technology

[0092]The hybridization array provides for analysis of the targets via hybridization, with the cali

Problems solved by technology

Insufficient stringency can result in a high degree of nonspecific binding.
Excessive stringency can lead to a failure of appropriate binding, which results in diminished sensitivity.
Despite the advantages that molecular diagnostic tests offer, the growth of this type of testing in the clinical laboratory has been slower than expected and remains a minor part of the practice of laboratory medicine.
This is primarily due to the complexity and costs associated with nucleic acid testing compared with tests based

Method used

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  • Genetic analysis loc with hybridization array with calibration chamber containing chamber with a blocked inlet spotted with reporter
  • Genetic analysis loc with hybridization array with calibration chamber containing chamber with a blocked inlet spotted with reporter
  • Genetic analysis loc with hybridization array with calibration chamber containing chamber with a blocked inlet spotted with reporter

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Embodiment Construction

[0227]This overview identifies the main components of a molecular diagnostic system that incorporates embodiments of the present invention. Comprehensive details of the system architecture and operation are set out later in the specification.

[0228]Referring to FIGS. 1, 2, 3, 130 and 131, the system has the following top level components:

[0229]Test modules 10 and 11 are the size of a typical USB memory key and very cheap to produce. Test modules 10 and 11 each contain a microfluidic device, typically in the form of a lab-on-a-chip (LOC) device 30 preloaded with reagents and typically more than 1000 probes for the molecular diagnostic assay (see FIGS. 1 and 130). Test module 10 schematically shown in FIG. 1 uses a fluorescence-based detection technique to identify target molecules, while test module 11 in FIG. 130 uses an electrochemiluminescence-based detection technique. The LOC device 30 has an integrated photosensor 44 for fluorescence or electrochemiluminescence detection (descri...

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Abstract

A microfluidic device having a supporting substrate, an inlet for receiving a biological sample containing target nucleic acid sequences, hybridization chambers in fluid communication with the inlet, the hybridization chambers containing probes that each have a nucleic acid sequence for hybridization with the target nucleic acid sequences to form probe-target hybrids, a fluorophore and a quencher configured such that the fluorophore emits a fluorescence signal in response to an excitation light and the quencher quenches the fluorescence signal when the probe is not hybridized, but fails to quench the fluorescence signal from the probe-target hybrid, and, a calibration chamber not in fluid communication the inlet, wherein, the calibration chamber contains a fluorophore.

Description

FIELD OF THE INVENTION[0001]The present invention relates to diagnostic devices that use microsystems technologies (MST). In particular, the invention relates to microfluidic and biochemical processing and analysis for molecular diagnostics.CO-PENDING APPLICATIONS[0002]The following applications have been filed by the Applicant which relate to the present application:GBS001USGBS002USGBS003USGBS005USGBS006USGSR001USGSR002USGAS001USGAS002USGAS003USGAS004USGAS006USGAS007USGAS008USGAS009USGAS010USGAS012USGAS013USGAS014USGAS015USGAS016USGAS017USGAS018USGAS019USGAS020USGAS021USGAS022USGAS023USGAS024USGAS025USGAS026USGAS027USGAS028USGAS030USGAS031USGAS032USGAS033USGAS034USGAS035USGAS036USGAS037USGAS038USGAS039USGAS040USGAS041USGAS042USGAS043USGAS045USGAS046USGAS047USGAS048USGAS049USGAS050USGAS054USGAS055USGAS056USGAS057USGAS058USGAS059USGAS060USGAS061USGAS062USGAS063USGAS065USGAS066USGAS067USGAS068USGAS069USGAS070USGAS080USGAS081USGAS082USGAS083USGAS084USGAS085USGAS086USGAS087USGAS088USGAS...

Claims

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Application Information

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IPC IPC(8): C40B40/00C12M1/34
CPCB01L3/5027Y10T436/25B01L3/502738B01L7/52B01L2200/10B01L2300/023B01L2300/024B01L2300/0636B01L2300/0654B01L2300/0883B01L2300/10B01L2300/1827B01L2400/0406B01L2400/0633B01L2400/0677B01L2400/0688F16K99/003F16K99/0036G01N27/223C12Q1/68Y10T436/107497Y10T436/173845Y10T436/143333Y10T436/11Y10T436/145555Y10T436/203332Y10T436/25375B01L3/502707Y10T137/0352Y10T137/0391Y10T137/1044Y10T137/206Y10T137/2076Y10T137/2202Y02A90/10
Inventor AZIMI, MEHDISILVERBROOK, KIA
Owner GENEASYS
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