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Methods for removal of microcystins and isolation of phycocyanin from cyanobacteria

a technology of phycocyanin and microcystin, which is applied in the direction of peptides, chemical/physical processes, water/sewage treatment by ion exchange, etc., can solve the problems of inability to produce large-scale quantities of this beneficial protein, and the method does not remove any microcystins that may already be present in the water

Active Publication Date: 2012-06-21
DESERT LAKE TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, accessing these benefits in a safe and economical way continues to be a challenge.
Although methods of removing Microcystis from mixtures of other algae such as of AFA have been developed, such methods do not remove any microcystins that may already be present in the water.
Similarly, although several methods have been employed to purify phycocyanin, it remains a challenge to produce large-scale, readily ingestible quantities of this beneficial protein.

Method used

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  • Methods for removal of microcystins and isolation of phycocyanin from cyanobacteria
  • Methods for removal of microcystins and isolation of phycocyanin from cyanobacteria
  • Methods for removal of microcystins and isolation of phycocyanin from cyanobacteria

Examples

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Effect test

example 1

Removal of Microcystins Contamination from a Blue-Green Algae Preparation

[0093]This example demonstrates the method of removing microcystins contamination from freshly-harvested AFA. This embodiment of the method is illustrated in FIG. 1.

1) AFA Preparation.

[0094]Freshly harvested AFA was collected in a 250 gallon plastic tote and frozen. The amount of algae collected was 1-5% dry weight, and was visibly contaminated with Microcystis aeruginosa. The contents of the tote were thawed to lyse the algal cells and release the water-soluble cellular constituents (such as phycocyanin and microcystin LR) into the aqueous extract. The suspended solids in the lysed cell mixture were removed by centrifugation, and the dissolved solid content of the supernatant was adjusted to 1.5-2.5% (dry weight) by either adding water or removing water by reverse osmosis.

2) Microcystin Removal.

[0095]AMBERLITE® FPX66 food grade adsorbent resin (Rohm and Hass) was packed into a column with a 20 liter bed volu...

example 2

Scaled-Up Removal of Microcystins Contamination from a Blue-Green Algae Preparation

[0097]This example illustrates a scaled-up method of removing microcystins contamination from a preparation of blue-green algae.

[0098]Removal of microcystins contamination from AFA can be scaled up to increase the amount of harvested cells that can be processed through a single column packed with a resin such as AMBERLITE FPX66. Cells are processed as in Example 1, except multiple plastic totes (for example, three) are processed simultaneously. Following removal of cellular debris, the supernatant of three totes is channeled into the column for microcystin removal. The post-column concentration of microcystins in the AFA preparation is measured by ELISA as in Example 2.

example 3

Removal of Microcystins Contamination from Water

[0099]The presence of Microcystis algae in sources of drinking water, and the resultant contamination of those sources with microcystins, is a global public health problem. The methods disclosed herein of removing microcystins from a blue-green algae preparation can also be applied in the context of removing microcystins contamination from any contaminated aqueous solution, including sources of drinking water. This example illustrates the removal of microcystins contamination from any water sample.

[0100]Removal of microcystins from contaminated water was achieved by the same method described in Example 1, except that the water does not need to be frozen prior to processing. In examples where the water contains few suspended solids, it is also not necessary to centrifuge the sample to be purified. Using the method described in Example 1, two liters of microcystins-contaminated water containing 0.25 ppm microcystin LR were passed through...

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Abstract

This disclosure relates to methods of removing contaminating microcystins toxins from preparations of blue-green algae. It also relates to methods of purifying phycocyanin from blue-green algae extracts.

Description

CROSS REFERENCE TO RELATED APPLICATION[0001]This application claims the benefit of U.S. Provisional Application No. 61 / 271,315, filed Jul. 20, 2009, which is incorporated by reference herein in its entirety.FIELD OF THE DISCLOSURE[0002]This disclosure relates to methods of removing contaminating microcystins toxins from preparations of blue-green algae. It also relates to methods of purifying phycocyanin from blue-green algae extracts.BACKGROUND[0003]The health benefits of a diet including blue-green algae such as Aphanizomenon flos aquae (AFA) and species of Arthrospira (commonly known as Spirulina) have long been appreciated. In particular, certain phytochemicals such as phycocyanin are well known for beneficial properties including antioxidant and anti-inflammatory capabilities (Shih et al., Anesthesia &Analgesia, 108:1303-1310, 2009). However, accessing these benefits in a safe and economical way continues to be a challenge.[0004]Blooms of harmful Microcystis aeruginosa, which p...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C07K14/405B01D15/12B01D15/26C07K1/14B01D15/08
CPCA23L1/3014C12N1/12A23L33/135
Inventor EHMANN, AXELGUTHRIE, JESSE
Owner DESERT LAKE TECH
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