Transgenic Plants Resistant To Non-Protein Amino Acids

a technology plants, applied in the field of transgenic plants, can solve the problems of inability to differentiate between phe and tyr, and inability to bind non-protein amino acids in proteins, etc., to prevent the incorporation of non-protein amino acids into proteins, and the plant is resistant to meta-tyrosine

Inactive Publication Date: 2014-08-21
YEDA RES & DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0013]The present invention is based in part on the unexpected discovery that expressing bacterial phenylalanyl-tRNA synthetase (PheRS) within a plant cell, particularly when the PheRS is expressed within the mitochondria and / or chloroplast, confers resistance of the plant to meta-tyrosine. This resistance is due to the ability of the introduced bacterial PheRS to hydrolyze the misacylated m-Tyr-tRNAPhe and to prevent the incorporation of the non-protein amino acid into proteins.

Problems solved by technology

It is therefore likely that in many cases, amino acids damaged in vivo are available for de-novo synthesis of proteins.
However, due to stereo-chemical similarity shared by several amino acids, mistakes in recognition occur.
Phenylalanine (Phe) and Tyrosine (Tyr) are distinguished by only one hydroxyl group at the aromatic ring and thus differentiation between Phe and Tyr is not always fulfilled.
Thus, incorporation of m-Tyr instead of Phe into organellar proteins results in a large number of damaged proteins, therefore reducing cell viability.
The shortage of the use of m-Tyrosine as a bioherbicide is that it is toxic not only to weeds but also to crop plants.

Method used

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  • Transgenic Plants Resistant To Non-Protein Amino Acids
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Examples

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example 1

The Effect of Bacterial PheRS Expression on Arabidopsis Resistance to m-Tyr

[0091]The bacterial PheRS genes described in the “Material and Methods” section hereinabove were expressed under the control of the constitutive 35S CaMV promoter. A transit peptide was appended to N-terminus of EcPheRS-α and EcPheRS-β subunits of the bacterial enzyme in order to direct them into the mitochondria and chloroplast of Arabidopsis thaliana. The second constructs pair including PheRS-α and PheRS-β lacked the transit peptides. Thus, four different constructs were transformed into Arabidopsis thaliana, and homozygote self-pollinated plants were generated as described hereinabove. Each line was further crossed to create plants containing heterodimeric EcPheRS possessing editing activity localized in cytoplasm (cyt-PheRS) and heterodimeric EcPheRS localized in plant mitochondria and chloroplast (mtp-PheRS). Several independent transgenic lines were obtained, and their resistance to m-Tyr was analyzed....

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Abstract

Transgenic plants resistant to bio-herbicides, particularly to phytotoxic non-protein amino acids including the meta-tyrosine (m-tyrosine) amino acid analog and salts thereof, means and methods for producing the transgenic plants.

Description

FIELD OF THE INVENTION[0001]The present invention relates to transgenic plants resistant to bio-herbicides, particularly to phytotoxic non-protein amino acids including meta-tyrosine amino acid analog and salts thereof and to methods for producing the transgenic plants.BACKGROUND OF THE INVENTION[0002]“Allelopathy” is a term referring to an effect (inhibitory or stimulatory) of a plant on surrounding species by chemicals (allelochemicals) released by the plant into the environment. The allelochemicals are usually secondary metabolites that can be synthesized in any of the plant parts, and can have beneficial (positive allelopathy) or detrimental (negative allelopathy) effects on the target organisms. Allelochemicals are not required for the metabolism (i.e. growth, development and reproduction) of the allelopathic (resistant) plant, but interfere with vital metabolic pathways of non-resistant species providing relative advantage to the resistant plant. The allelopathic effect was re...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C12N15/82
CPCC12N15/8251C12N9/93C12N15/821C12N15/8274
Inventor SAFRO, MARKKLIPCAN, LIRONMAYMON, INBARFINAROV, IGAL
Owner YEDA RES & DEV CO LTD
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