Methods and devices suitable for imaging blood-containing tissue
a blood-containing tissue and imaging technology, applied in the field of medical imaging, can solve the problems of insufficient contrast in visible-light color images to clearly distinguish small blood vessels
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example 1
[0170]A first laboratory mouse was anesthetized. The skin covering the abdominal cavity was cut to define a loose flap, and the flap secured with needles to a flat surface, skin side down.
[0171]Using an SD-300 spectral imaging camera (ASI, Migdal Haemek, Israel) fitted with a halogen lamp illuminator and a cross-polarizing filter set, a spectral image of a portion of the inner surface of the flap was acquired between 400 nm and 800 nm. The acquired image is reproduced, in black-and-white, in FIG. 1A. In FIG. 1B, the spectrum of a portion of the image where a blood vessel was present (portion a) and the spectrum of the image where no blood vessel was present (portion b) are displayed.
example 2
[0172]A second laboratory mouse was anesthetized. The skin covering the abdominal cavity was cut to define a loose flap, and the flap secured with needles to a flat surface, skin side down.
[0173]Using an SD-300 spectral imaging camera (ASI, Migdal Haemek, Israel) fitted with a halogen lamp illuminator and a cross-polarizing filter set, a spectral image of a portion of the inner surface of the flap was acquired between 400 nm and 800 nm.
[0174]An RGB image including all the image data acquired between 400 nm and 800 nm is reproduced in black-and-white in FIG. 2A.
[0175]A red-free narrow band (520 nm-580 nm) monochrome image using data acquired between is reproduced in black-and-white in FIG. 2B. Although the contrast is superior to the equivalent RGB image, the spatial resolution appears to be similar.
[0176]An image generated in accordance with an embodiment of the method described herein where the first wavelength range was 400 nm to 600 nm and the second wavelength range was 600 nm t...
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