Curcumin-peptide conjugates and formulations
a technology of curcumin and peptides, which is applied in the field of nutraceutical formulations, can solve the problems of curcumin's poor solubility, limited in vivo suitability for in vivo studies, and curcumin's poor solubility, and reduces the bioavailability of curcumin throughout the body
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example 1
Preparation of Curcumin-Whey Protein Isolate Complex
[0047]A curcumin-WPI complex was prepared for administration to human subjects. The following materials were used:
WPI:85% protein by weightCurcumin powder:95% curcuminoidsEthanol:100% ethyl alcohol
[0048]Ratio of curcumin:WPI of 1:50 w / w. A 0.5% w / v tincture (solution) was prepared by mixing 5 g curcumin powder with 1000 mL ethanol. The mixture was placed on a magnetic stirring hot plate, with a speed setting at medium, and temperature setting at 50° C. for 30 minutes or until solution turned clear. To the resulting solution was added 250 g WPI powder. The mixture was placed on a rotary evaporator (rotovap) at slow speed (20-30 rpm), having a water bath temperature of 50° C., and low vacuum for 30 minutes, or until 90% of the ethanol was evaporated. The resulting mixture was placed in a kiln oven to remove the remainder of the ethanol. Alternatively, the mixture was placed in a lyophilizer, or alternatively, was placed in a flow hoo...
example 2
Preparation of Curcumin-Amino Acid Complexes
[0052]Following the method of Example 1, curcumin was dissolved in 95% ethanol, followed by the addition of N-acetyl cysteine (NAC). An escalating ratio of the concentrations of curcumin to n-acetyl cysteine was used to find the saturation level. The optimal concentration was found at around 100 mg of curcumin to 1000 mg of NAC in a 0.5% (w / v) solution of curcumin in ethanol. Optimal concentration was noted when low number of Curcumin crystal formation was observed in the macroscopic field.
[0053]Similarly, curcumin was dissolved in 95% ethanol, followed by the addition of cysteine. An escalating ratio of the concentrations of curcumin to cysteine was used to find the saturation level. The optimal concentration was found at around 100 mg of curcumin to 1000 mg of cysteine in a 0.5% (w / v) solution of curcumin in ethanol. Optimal concentration was noted when low number of Curcumin crystal formation was observed in the macroscopic field.
[0054]...
example 3
Administration of Curcumin:WPI Complex
[0056]Two healthy individuals were administered a single dose of the curcumin:WPI complex, having a ratio of curcumin:WPI of 25:1 w / w (mg:g). The dosage contained 25 mg of curcumin. Blood was drawn from each individual at 20 min, 50 min, and 90 min, and the level of serum curcumin was calculated. The results are shown in Table 1, below.
TABLE 1Subject ATime (min)205090Avg (ng / mL)1426.81162.0212.7StDev1200.0932.551.1Subject BTime (min)305090Avg (ng / mL)2716.62011.2508.4StDevN / A997.6284.6
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