Strong cation exchange chromatographic matrix and method for using same
a chromatographic matrix and cation exchange technology, applied in the field of ion exchange chromatographic matrix, can solve the problems of long separation time, complex operation, and difficulty in purification, and achieve the effect of efficient flow-through purification
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Benefits of technology
Problems solved by technology
Method used
Image
Examples
example 1
[0131]In Example 1, a cation exchange membrane in a hollow fiber form was prepared by the radiation graft polymerization method.
[0132]1) Preparation of Cation Exchange Membrane
[0133]7.07 g of 2-hydroxyethyl methacrylate and 0.79 g of glycidyl methacrylate were dissolved in 113 mL of methanol, and the solution was bubbled with nitrogen for 30 minutes and used as a reaction solution. 3.03 g (15 cm, 15 fibers) of polyethylene porous hollow fibers having an outside diameter of 3.0 mm, an inside diameter of 2.0 mm, and an average pore size of 0.25 um was placed in a closed container, and air in the container was replaced with nitrogen. Then, the container was irradiated with 25 kGy of γ ray while cooled on dry ice from the outside to generate radicals. The polyethylene porous hollow fibers having the obtained radicals were transferred to a glass container, and oxygen in the reaction tube was removed by reduction in pressure to 200 Pa or lower. 55 mL of the reaction solution adjusted to 4...
example 2
[0154]In Example 2, the same operation as in Example 1 was performed except that a different buffer solution (15 mmol / L acetate buffer solution of pH 5.0 containing 30 mmol / L sodium chloride) was used in the antibody solution and the washing step. The amount of the antibody solution treated was 50 mL, and the concentration thereof was 5.56 mg / mL. The results are shown in FIG. 3.
example 3
[0155]In Example 3, the same operation as in Example 2 was performed except that a different buffer solution (15 mmol / L acetate buffer solution of pH 5.0 containing 50 mmol / L sodium chloride) was used in the antibody solution and the washing step. The amount of the antibody solution treated was 40 mL, and the concentration thereof was 5.56 mg / mL. The results are shown in FIG. 3.
PUM
| Property | Measurement | Unit |
|---|---|---|
| Fraction | aaaaa | aaaaa |
| Fraction | aaaaa | aaaaa |
| Fraction | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More