Medium for producing glucosamine
a technology of glucosamine and enzymatic hydrolysis, which is applied in the direction of organic chemistry, peptides, chemical apparatus and processes, etc., can solve the problems of acid waste treatment, low enzymatic hydrolysis efficiency, and the inability to commercialize enzymatic production of glucosamine, so as to reduce the amount of metal ions, inhibit fungal growth, and reduce the yield of glucosamine
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example 1
Effects of Pretreatment of Molasses on Fungal Growth
[0043]Molasses, a major by-product of the cane sugar industry, is dark-brown, thick liquid rich in nutrients. However, due to the equipment used in refining process of sugar cane, molasses stock is usually contaminated by a high amount of metal ions, which inhibits fungal growth and further reduces yields of glucosamine. Therefore, pretreatment of molasses stock may be important for glucosamine production, and in this Example, comparison of effects of untreated molasses stock and treated molasses on fungal growth was carried out.
[0044]I. Pretreatment of Molasses
[0045]Molasses stock was mixed with water at a ratio of 1:1 by volume, and the mixture was stirred until molasses stock was completely dissolved. The mixture was then stored at 4° C. to settle for 24 hours. After that, two layers were formed. The upper layer was a dark brown liquid, and the lower layer was brown mud. The volume ratio of the upper layer and the lower layer wa...
example 2
Effects of Concentrations of Molasses on Fungal Growth
[0051]I. Fungal Fermentation Test
[0052]Aspergillus sydowii BCRC 31742 (Hsinchu, Taiwan) was also used in the tests of this Example. First, the fungi were recovered by being subcultured twice with the method described in Example 1.
[0053]After that, 15 mL of the recovered fungi were seeded in different flasks each containing 150 mL of the following media, respectively:
(1) M200Sb5AlMe liquid medium [200 mL / L molasses (equivalent to about 110.8 g / L molasses), 5 mL / L soybean hydrolysate (equivalent to about 2.81 g / L soybean hydrolysate), 0.1 g / L MgSO4.7H2O, 0.1 g / L Al(NO3)3, and 1 mL / L methanol, pH 7]; and
(2) M300Sb5AlMe liquid medium [300 mL / L molasses (equivalent to about 163 g / L molasses), 5 mL / L soybean hydrolysate (equivalent to about 2.81 g / L soybean hydrolysate), 0.1 g / L MgSO4.7H2O, 0.1 g / L Al(NO3)3, and 1 mL / L methanol, pH 7].
The fungi were incubated at 30° C., 200 rpm for 5 days, and then were subject to determination of gluc...
example 3
[0058]Effects of Concentrations of Aluminum Nitrate (Al(NO3)3) on Fungal Growth
[0059]I. Fungal Fermentation Test
[0060]Aspergillus sydowii BCRC 31742 (Hsinchu, Taiwan) was also used in the tests of this Example. First, the fungi were recovered by being subcultured twice with the method described in Example 1.
[0061]After that, 15 mL of the recovered fungi were seeded in different flasks each containing 150 mL of the following media, respectively:
(1) M300Sb5Me liquid medium [300 mL / L molasses (equivalent to about 163 g / L molasses), 5 mL / L soybean hydrolysate (equivalent to about 2.81 g / L soybean hydrolysate), 0.1 g / L MgSO4.7H2O, 0 g / L Al(NO3)3, 1 mL / L methanol, pH 7];
(2) M300Sb5AlMe liquid medium [300 mL / L molasses (equivalent to about 163 g / L molasses), 5 mL / L soybean hydrolysate (equivalent to about 2.81 g / L soybean hydrolysate), 0.1 g / L MgSO4.7H2O, 0.1 g / L Al(NO3)3, 1 mL / L methanol, pH 7];
(3) M300Sb5Al2Me liquid medium [300 mL / L molasses (equivalent to about 163 g / L molasses), 5 mL / ...
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