Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Differentiation of lyme disease and southern tick-associated rash illness

a technology of southern ticks and differential markers, applied in the field of human disease detection tools and methods, can solve the problems that i>b. burgdorferi /i>antibodies cannot be used as reliable differential markers for stari, no laboratory tools or methods exist for diagnosis, and poor sensitivity

Pending Publication Date: 2022-06-02
COLORADO STATE UNIV RES FOUND +1
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The present patent describes a method for analyzing a blood sample from a subject with Lyme disease or STARI. The method involves deproteinizing the blood sample to produce a metabolite extract, and then performing liquid chromatography coupled to mass spectrometry on the metabolite extract. The resulting data is then input into a classification model trained with samples of metabolite extracts from suitable controls. The model produces a disease score that distinguishes subjects with Lyme disease or STARI from healthy subjects. The method can be used for diagnosis and treatment of the diseases.

Problems solved by technology

Clinically, the skin lesions of STARI and early Lyme disease are indistinguishable, and no laboratory tool or method exists for the diagnosis of STARI or differentiation of STARI from Lyme disease.
However, these tests have poor sensitivity for early stages of Lyme disease, and thus a lack of B. burgdorferi antibodies cannot be used as a reliable differential marker for STARI.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Differentiation of lyme disease and southern tick-associated rash illness
  • Differentiation of lyme disease and southern tick-associated rash illness
  • Differentiation of lyme disease and southern tick-associated rash illness

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0085]Lyme disease is a multisystem bacterial infection that in the United States is primarily caused by infection with Borrelia burgdorferi sensu stricto. Over 300,000 cases of Lyme disease are estimated to occur annually in the United States, with over 3.4 million laboratory diagnostic tests performed each year (1, 2). Symptoms associated with this infection include fever, chills, headache, fatigue, muscle and joint aches, and swollen lymph nodes; however, the most prominent clinical manifestation in the early stage is the presence of one or more erythema migrans (EM) skin lesions (3). This annular, expanding erythematous skin lesion occurs at the site of the tick bite in 70 to 80% of infected individuals and is typically 5 cm or more in diameter (4, 5). Although an EM lesion is a hallmark for Lyme disease, other types of skin lesions can be confused with EM (3, 5, 6). These include rashes caused by tick-bite hypersensitivity reactions, certain cutaneous fungal infections, bacteri...

example 2

[0108]STARI is an illness that has received little attention over the years, but is a confounding factor in diagnosing early Lyme disease in areas where both illnesses overlap and contributes to the debate surrounding the presence of Lyme disease in the southern United States. No diagnostic tool exists for STARI or for differentiating early Lyme disease from STARI. Based on documented differences between early Lyme disease and STARI (9, 16, 56), we metabolically profiled serum to develop a biochemical biosignature that when applied could accurately classify early Lyme disease and STARI patients (See Example 1). This example describes the design of the study described in Example 1.

[0109]An unbiased-metabolomics study was designed to directly compare the metabolic host responses between these two illnesses, and subsequently evaluate how this metabolic biosignature distinguishes these two illnesses. The use of unbiased metabolomics for biosignature discovery does not lend itself to pow...

example 3

[0113]This example describes methods used for Lyme disease serologic testing of all serum samples used in the examples above. Standard two-tiered testing was performed on all samples (38). The C6 B. burgdorferi (Lyme) ELISA (Immunetics, Boston, Mass.) was used as a first-tier test, and any positive or equivocal samples were reflexed to Marblot IgM and IgG immunoblots (MarDx Diagnostics, Inc., Carlsbad, Calif.) as the second-tier test. Serologic assays were performed according to the manufacturer's instructions, and the data were interpreted according to established CDC guidelines (38). Duration of illness, however, was not considered for test interpretation.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present disclosure provides a biosignature that distinguishes Lyme disease, including early Lyme disease, from STARI. The present disclosure also provides methods for detecting Lyme disease and STARI, as well as methods for treating subjects diagnosed with Lyme disease or STARI.

Description

CROSS REFERENCE TO RELATED APPLICATIONS[0001]This application is a continuation application of U.S. application Ser. No. 16 / 703,401, filed Dec. 4, 2019, which application is a continuation of International Application No. PCT / US2018 / 036688, with an international filing date of Jun. 8, 2018, which PCT application claims the benefit of U.S. provisional application No. 62 / 516,824, filed Jun. 8, 2017. The contents of the above-mentioned applications are hereby incorporated by reference in their entirety.GOVERNMENTAL RIGHTS[0002]This invention was made with government support under AI100228 and AI099094, each awarded by National Institutes of Health. The government has certain rights in the invention.REFERENCE TO SEQUENCE LISTING[0003]The instant application contains a Sequence Listing which has been submitted electronically in ASCII format and is hereby incorporated by reference in its entirety. Said ASCII copy, created on Apr. 27, 2021, is named “CSURF_065620-642266_ST25.txt”, and is 2...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(United States)
IPC IPC(8): C12Q1/04G01N33/68
CPCC12Q1/04G01N33/6848G01N2800/26G01N2800/20G01N2333/20G01N33/56911G01N2560/00Y02A50/30
Inventor BELISLE, JOHN T.MOLINS, CLAUDIA R.WORMSER, GARY P.
Owner COLORADO STATE UNIV RES FOUND
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com