Supercharge Your Innovation With Domain-Expert AI Agents!

Trioxyethylene gold nanoclusters functionalized with a single DNA

a technology of trioxyethylene gold and nanoclusters, applied in the field of trioxyethylene gold nanoclusters functionalized with a single dna, can solve the problems of cumbersome and low isolation use throughput, and achieve the effect of rapid scaling

Inactive Publication Date: 2010-02-02
NAVY USA AS REPRESENTED BY THE SEC OF THE THE
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This method produces highly stable, charge-neutral nanoclusters suitable for quantitative detection and bio-molecular-directed self-assembly, enabling precise positioning and large-scale production of nanoclusters with a DNA-to-nanocluster ratio approaching 1:1, suitable for electronic devices and diagnostics.

Problems solved by technology

The method does not rely on the gel electrophoresis technique, which is cumbersome and low-throughput for isolation uses.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Trioxyethylene gold nanoclusters functionalized with a single DNA
  • Trioxyethylene gold nanoclusters functionalized with a single DNA
  • Trioxyethylene gold nanoclusters functionalized with a single DNA

Examples

Experimental program
Comparison scheme
Effect test

example

[0025]A 15-mer DNA oligomer (5′ HO—(CH2)6—S—S—(CH2)6-ACACACACACACACA-Fluorecein-3′ (SEQ. ID. NO.: 1)) was synthesized and HPLC purified. The disulfide of the 5′-terminus was first reduced with a freshly prepared 75 mM dithiothreitol solution and the resulting mono-thiolated oligonucleotide was purified on a 15% acrylamide gel. The oligonucleotide from the excised gel slices was eluted into 1 mL distilled, deionized water, (R=18 M·Ω·cm−1). The concentration of the DNA was determined by measuring A260 using a molar extinction coefficient provided by the manufacturer. Prior to DNA-conjugation, the solution of AuEO3 was subjected to further purification on a P-6 size exclusion column to remove any residual EO3SH or AuEO3 with residual alkane thiol (which were insoluble in water). The concentration of the AuEO3 solution was estimated using a molar extinction coefficient of 5×105 L·mol−1·cm−1. This value was obtained by measuring an extinction coefficient at 512 nm of 9.7 L·g−1·cm−1 for a...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
sizeaaaaaaaaaa
lengthaaaaaaaaaa
pHaaaaaaaaaa
Login to View More

Abstract

A method of making a nanoclusters functionalized with a single DNA strand comprising the steps of providing nanoclusters, combining said nanoclusters with thiolated DNA, incubating said nanoclusters and thiolated DNA mixture, combining said mixture with a solution comprising ethanol and dichloromethane; separating said mixture into an aqueous phase and an organic phase, mixing said aqueous phase with a solution comprising dicholormethane and NaCl, and separating the mixture into an aqueous phase and an organic phase; wherein said organic phase comprises said nanoclusters functionalized with a single DNA strand. Further, provided is a nanocluster functionalized with a single DNA strand comprising a nanocluster, said nanocluster being functionalized with a single DNA strand, said DNA strand having a length of about 10 to about 50 bases.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS[0001]This Application is a Continuation-in-Part of U.S. application Ser. No. 10 / 206,431, now issued as U.S. Pat. No. 7,404,928, filed Jul. 29, 2002 and a Non-Prov of Prov (35 USC 119(e)) application Ser. No. 60 / 668,876 filed on Apr. 1, 2005, both incorporated herein in their entirety.BACKGROUND OF THE INVENTION[0002]Gold nanoclusters (GNCs) have received been considered as potential building blocks for a variety of nanoscale applications, including chemical sensing, electronics, optics, and biology. A scalable method for isolating significant quantities of GNCs with a known number of DNA strands per GNC would be useful in nanoscale applications dependent on using DNA molecular recognition in patterned self-assembly scemes, as DNA, with its highly specific base-pairing is attractive as the basis of self-assembly of nanoclusters. Earlier work demonstrated the feasibility of this approach using GNCs coupled with multiple oligonucleotides; but to ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(United States)
IPC IPC(8): G01N21/75B22F1/054B22F1/102
CPCA61K47/48861A61K47/48884B22F1/0018B22F1/0062B82Y15/00B82Y30/00B01J2219/0063B01J2219/00637A61K47/6923A61K47/6929B22F1/102B22F1/054
Inventor JHAVERI, SULAYANCONA, MARIOFOOS, EDWARD ECHANG, EDDIE L
Owner NAVY USA AS REPRESENTED BY THE SEC OF THE THE
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com