Scierotomal cell amplifying bioreactor system

A technology of bioreactor and osteoblast, which is applied in the system field of bioengineering technology, can solve the problems of uneconomical device design, incapable of multi-cell capacity, and unfavorable saving of medium, so as to prevent the generation of air bubbles and benefit The effect of diffusion, stable and efficient amplification

Inactive Publication Date: 2007-09-19
SHANGHAI JIAOTONG UNIV
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

The device cannot flexibly adjust the way of perfusion (circulation or one-way), which is not conducive to saving medium, and cannot obtain the maximum amount of cells with the minimum amount of medium supply
In addition, this device uses the perfusion method of the medium from top to bottom instead of the reverse method, which is not conducive to the thorough mixing of the medium, and it is not easy to fully exhaust the air bubbles at the beginning of the perfusion, and this device is not easy for the medium sampling
All in all, there are limitations in the design of the system, the design of the device is uneconomical, the design of the inflow of the medium is unreasonable, and it is impossible to take out the medium at any time while culturing the cells to analyze its components and predict the growth state of the cells

Method used

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  • Scierotomal cell amplifying bioreactor system

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Embodiment Construction

[0018] As shown in Figure 1, the present invention comprises: cell culture box 1, liquid storage tank 2, the first microporous membrane filter 3, peristaltic pump 7, the first four-way pipe 8, sealing cover 9, perfusion chamber 12, support 13. Second four-way pipe 14, third four-way pipe 15, first stop clip 16, second stop clip 17, third stop clip 18, waste liquid tank 19, second microporous membrane filter twenty two.

[0019] The liquid storage tank 2 is connected to the first four-way pipe 8 through a silicone tube, the peristaltic pump 7 is in the middle of the silicone tube connecting the liquid storage tank 2 and the first four-way pipe 8, and the perfusion chamber 12 is connected to the first four-way pipe 8 through a silicone tube. It is connected with the second four-way pipe 14, the sealing cover 9 is covered on the perfusion chamber 12, the bracket 13 is placed in the perfusion chamber 12, the liquid storage tank 2 is connected with the third four-way pipe 15 throug...

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Abstract

This invention relates to a scierotomal cell amplification bioreactor system, in which, a liquor storage tank is connected with a first double tee via a silica-gel tube with a peristaltic pump in it, an injection chamber is connected with a first and second double tee via the silica-gel tube, a sealing cover covers the injection chamber containing a frame, the liquor storage tank is connected with a third double tee tube via a silica-gel tube with a first stop clamp set on it, the third double tee is linked with a waste liquor tank via a silica-gel tube, a third stop clamp is put on it, the third double tee tube is linked with a section of a silica gel tube with a second stop clamp on it, the storage tank is connected with a first micro hole film filter, the waste liquor tank is linked to a second micro hole film filter, all of them are set in a cell incubator.

Description

technical field [0001] The invention relates to a system in the technical field of bioengineering, in particular to an osteoblast expansion bioreactor system. Background technique [0002] Bone tissue engineering is used for the repair and reconstruction of bone defects, and has significant advantages over traditional methods: (1) not limited by donor sources; (2) avoiding immune rejection in allogeneic tissue transplantation; (3) artificial biological The shape and size of the material can be designed and shaped according to different tissues and actual needs, without being limited by the shape of the donor; the ultimate goal is to provide an ideal bioactive bone tissue with a wide range of sources, easy application, and definite curative effect. Osteoblasts are one of the basic elements in the formation of tissue engineered bone tissue, and the expansion and culture of osteoblasts in vitro is an important aspect of tissue engineering in the study of bone defects. Since th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00C12M3/00
CPCC12M23/00
Inventor 董良齐瀚实陈彦田沈阳孙海英
Owner SHANGHAI JIAOTONG UNIV
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