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IHNV G protein for immune stimulation

A protein and protein technology, applied in veterinary vaccines, DNA/RNA fragments, vaccines, etc., can solve problems such as displaying fish protection

Inactive Publication Date: 2007-09-26
ELANCO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it has been difficult to convincingly show protection in fish using NAVs based on other viral antigens

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1: Evaluation of a nucleic acid vaccine against infectious pancreatic necrosis virus in Atlantic salmon Salmo salar

[0049] vaccination

[0050] Juvenile Atlantic salmon (body weight 9-26 g) were placed in two 1 meter diameter circular tanks filled with fresh water at a temperature of 8°C and starved for 24 hours before inoculation. For vaccination, fish were anesthetized in ethyl 3-aminobenzoate (MS222, Sigma, Poole, UK) at a concentration of about 0.5 g / liter. The nucleic acid vaccine was administered by intramuscular injection (10 μg DNA / 50 μl dose) in the region under the dorsal fin on the left dorsal side. Oil-assisted inactivated IPNV vaccine and PBS control were administered by intraperitoneal injection (100 μl). There were 40 fish per treatment group and two replicates for each of the 6 vaccines.

[0051] The test group received the following compositions:

[0052] (1) pUK vector: a cloning vector carrying kanamycin resistance gene, CMV immediate ea...

Embodiment 2

[0068] Example 2: Evaluation of nucleic acid vaccines against ISAV

[0069] Juvenile Atlantic salmon (<6 months old) weighing an average of 10 g are acclimatized for at least 7 days in water flowing at 2.5 L / min at 12±1°C. Fish were fed a commercial pelleted diet at a rate of 1.5% of body weight per day.

[0070] Fish were anesthetized in 30 mg / l benzocaine prior to vaccination. The nucleic acid vaccine was administered by intramuscular injection (10 μg DNA / 50 μl dose) in the postaxial muscle in front of the dorsal fin. The oil emulsion vaccine was administered by intraperitoneal injection (150 μl). Each replicate vaccine group had 55 fish and was replicated twice for each of the 5 vaccines.

[0071] The test group received the following compositions:

[0072] (1) pUK vector: a cloning vector carrying kanamycin resistance gene, CMV immediate early promoter, multiple cloning sites and bovine auxin polyadenylation signal (BGH polyA).

[0073] (2) pUK-HA: a pUK vector in whi...

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PUM

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Abstract

A vaccine comprises a portion of IHNV G protein and a portion of a second protein from a fish pathogen, or their respective nucleic acid coding sequences. The presence of the IHNV G protein boosts the immune response to the second protein, resulting in a protective effect against infection by the fish pathogen from which the second protein is derived.

Description

field of invention [0001] The present invention relates to the use of a new vaccine for preventing infectious diseases, especially the use of a new vaccine for preventing infectious diseases in fish. The present invention also relates to such a vaccine, which comprises a part of nucleic acid sequence encoding rhabdovirus such as IHNV (Infectious Haematopoietic Necrosis Virus (Infectious Haematopoietic Necrosis Virus)) G protein and a part of nucleic acid sequence encoding a second peptide, wherein said The second peptide comes from a pathogen. Background of the invention [0002] Recombinant vaccines are sometimes used in human and veterinary medicine as an alternative to more traditional methods based on inactivated or attenuated pathogens. Multiple exogenous antigens delivered systemically to the body in this manner activate only one pathway of the immune system, namely the production of antibodies by the major histocompatibility complex (MHC) class II pathway by stimulat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/295A61K39/205A61K39/12A61K39/145C12N15/85C12N15/62C07K14/145
CPCC12N2760/20034C12N2760/20022A61K39/145A61K39/12A61K39/295A61K39/205A61K2039/53C07K2319/02C12N2720/10022A61K2039/55516C07K14/005A61K2039/552A61K2039/55566C12N2720/10034C12N2760/16034C12N2760/20043A61P31/00A61P31/04A61P31/10A61P31/12A61P33/02C07K14/145C12N15/85C12N2800/106
Inventor N·C·西马德L·M·布特兰德
Owner ELANCO
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