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Use of ocean green algae in extraction of cyclic peptide components with anti-tumor activity

A technology of anti-tumor activity and marine green algae, which is applied in the application field of unicellular multinucleated marine green algae in the extraction of anti-tumor active cyclic peptide components, which can solve the limitation of preparation and utilization of cyclic peptide compounds, limited sea squirt and sponge resources , Breeding difficulties and other problems, to achieve the effect of low cost, good applicability and simple process

Inactive Publication Date: 2008-06-25
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As far as more than 200 cyclic peptide compounds have been identified in marine organisms, they are mainly derived from ascidians, sponges, molluscs, cyanobacteria and microorganisms. Most of the compounds are derived from molluscs ascidians and sponges, but the resources of ascidians and sponges limited and difficult to breed, thus limiting the preparation and utilization of cyclic peptide compounds

Method used

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  • Use of ocean green algae in extraction of cyclic peptide components with anti-tumor activity
  • Use of ocean green algae in extraction of cyclic peptide components with anti-tumor activity
  • Use of ocean green algae in extraction of cyclic peptide components with anti-tumor activity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] see figure 1 Shown, the preparation process of Bryopsis sp. cyclic peptide fraction.

[0021] Fresh Bryopsis sp., washed 2-3 times quickly with distilled water, squeezed dry, weighed about 1kg; reflux extracted with 2L methanol for 3 times, each 2h, filtered, combined the methanol filtrate obtained 3 times, concentrated under reduced pressure to volume 1 ~1.5L, and then sequentially extracted with equal volumes of n-butanol and chloroform; the n-butanol layer was washed 1-2 times with equal volumes of distilled water, and the n-butanol layer was taken by standing and separated, concentrated under reduced pressure, and dried in vacuo to form components 1. After the chloroform layer was concentrated to dryness under reduced pressure, add 1 L each of methanol-water (volume ratio 9:1) and n-hexane. The layers were separated; the chloroform layer was concentrated under reduced pressure and dried in vacuo to obtain component 2.

[0022] Using ninhydrin as the chromogenic a...

Embodiment 2

[0036] Take fresh moss feather algae (Bryopsis hypnoides), wash them quickly with distilled water twice, squeeze dry, and weigh about 1kg; each time extract with 3L ethanol for 3 times, each 2h, filter, combine the ethanol filtrates obtained three times, and concentrate under reduced pressure to The volume is 1-1.5L, and then extracted with an equal volume of n-butanol and chloroform in sequence. The n-butanol layer was washed with an equal volume of distilled water for 1-2 times, and the n-butanol layer was taken, concentrated under reduced pressure, and dried under vacuum to obtain component 1. After the chloroform layer was concentrated to dryness under reduced pressure, 2 L of methanol-water (volume ratio 8.5:1.5) and 1 L of n-hexane were added, and the methanol layer was taken after standing to separate layers, and the concentration of methanol was adjusted to 80%, and then an equal volume of chloroform was added to statically The layers were separated; the chloroform lay...

Embodiment 3

[0038] Take fresh Bryopsis plumosa, wash it twice quickly with distilled water, squeeze dry, and weigh about 1kg; extract twice with 2.5L ethanol each time, 4h each, filter, combine the ethanol filtrate obtained twice, and depressurize Concentrate to a volume of 1-1.5 L, and then extract with equal volumes of n-butanol and chloroform in sequence; the n-butanol layer is washed once with equal volumes of distilled water, and the n-butanol layer is taken, concentrated under reduced pressure, and vacuum-dried to obtain component 1. After the chloroform layer was concentrated to dryness under reduced pressure, add 0.8 L each of methanol-water (volume ratio 8.5:1.5) and n-hexane. After standing to separate, take the methanol layer, adjust the methanol concentration to 80%, and then add an equal volume of chloroform The layers were separated at rest; the chloroform layer was concentrated under reduced pressure and dried in vacuo to obtain component 2.

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Abstract

The invention relates to cyclic peptides components for antineoplastic activity, specially a unicellular multinuclear marine chlorophta for extracting cyclic peptides components for antineoplastic activity; the advantages of the invention is: easily-got raw material, rich resource; slight toxic side effect of the components, good antieoplastic activity; simple preparation technology; low cost; broad development potential and good applicability.

Description

technical field [0001] The invention relates to an anti-tumor active cyclic peptide component, in particular to the application of a single-cell multinuclear marine green algae in extracting an anti-tumor active cyclic peptide component. Background technique [0002] Cancer, cardiovascular disease, and AIDS are the three major diseases that threaten human health, and are therefore the three most popular diseases in global research. Most of the drugs currently used for these three types of diseases are chemically synthesized drugs, which have extremely toxic and side effects, and a particularly ideal synthetic drug has not been obtained so far. It is of great significance to screen compounds with anti-tumor and anti-viral activities from natural drug-derived organisms, and then chemically modify or semi-synthesize them into natural drugs and drug sources with unique curative effects. [0003] Cyclic peptides refer to a class of cyclic natural small molecular compounds mainly...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/405A61P35/00
Inventor 王广策王发左叶乃好刘海航曾呈奎
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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