Flow type cell detection method of aerobic non-oxygen-production photosynthetic heterotrophy bacterium
A technology of heterotrophic bacteria and flow cytometry, applied in the field of bacterial detection, can solve the problems of biomass estimation error, influence of AAPB function, AAPB quantitative error and so on.
A technology of heterotrophic bacteria and flow cytometry, applied in the field of bacterial detection, can solve the problems of biomass estimation error, influence of AAPB function, AAPB quantitative error and so on.
CN100396787CInactive Publication Date: 2008-06-25XIAMEN UNIV
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[0033] The following embodiments will further illustrate the present invention in conjunction with the accompanying drawings.
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The present invention relates to bacteria detection, and flow cytometer detecting process of aerobic anoxygenic photoheterotrophic bacteria (AAPB). The detection process includes the following steps: adding formaldehyde to the sample and culturing; staining the sample with DAPI, collecting forward and lateral scattered light, DAPI fluorescence signal, chlorophyll fluorescence signal and bacterial chlorophyll-a fluorescence signal and separating AAPB from other planktons by means of the difference of signals; separating planktons from other matters by means of DAPI fluorescence signals; separating panktobacteria from other micro planktons by means of the strength of forward and lateral scattered light; eliminating the influence of other matters on the AAPB detection by means of the strength of chlorophyll fluorescence signal; and separating AAPB from other bacteria by means of bacterial chlorophyll-a and obtaining the accurate number of AAPB.
Description
technical field [0001] The present invention relates to the detection of a kind of bacterium, especially relates to the detection of a type of marine ultramicrobial organisms—Aerobic Anoxyenic Phototrophic Bacteria (AAPB), through combining with flow cytometry, establishing A rapid detection method for aerobic anoxygenic photoheterotrophic bacteria. Background technique [0002] Discovered more than 20 years ago (Shiba T, Simidu U, Taga N. Distribution of aerobic bacteria which containbacteriochlorophyll a. Appl Environ Microbiol, 1979, 38: 43-45) is a class of aerobic non-oxygenous photoheterotrophic bacteria with different Aerobic heterotrophic bacteria function in oxygenic photosynthesis, but until recently their important role in marine ecosystems has not received much attention (Kolber Z S, Plumley F G, Lang A S, et al. Contribution of aerobicphotoheterotrophic bacteria to the carbon cycle in the ocean. Science, 2001, 292: 2492-2495), AAPB, as an important part of the ...
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Patent Timeline
25 Jun 2008
Publication
CN100396787C
- IPC
- C12Q1/04
- Inventors
- 焦念志; 骆åºä¼Ÿ