Method for improving vinblastine content in madagascar periwinkle
A technology of vinblastine and vinblastine, which is applied in the field of increasing the content of vinblastine in vinca, can solve the problems of difficult industrial production by chemical synthesis methods, and achieve the effects of easy industrial production, easy operation, and good natural activity
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Embodiment 1
[0020] Divide 10kg of freshly collected periwinkle flowers with a water content of 70-80% into 4 equal parts, place them in a beater, add 10kg of culture solution dissolved with an inducer, refine the pulp for 3 minutes, and the final particle size of the material is 0.02-8μm . Then pour it into a culture tank with a filter membrane, use negative pressure 0.06-0.1MPa suction or positive pressure 0.1-0.6MPa air intake to carry out homogenate culture every 2h, and each culture time is 10min. After culturing for 24 hours, the content of vinca alkaloids in the raw materials was determined by HPLC. The results showed that the content of vinblastine in the raw materials increased by 55%, and the content of vincristine increased by 65%.
[0021] In this example, the formula of culture solution is as follows:
[0022] (1) The culture medium is pure water;
[0023] (2) The formula of the inducer is:
[0024] Sodium acetate 0.02μmol / L
[0025] Ethephon 1mg / L
[0026] Abscisic acid ...
Embodiment 2
[0036] Divide 50 kg of periwinkle flowers with a water content of 60% for 3 days after collection into 10 equal parts, place them in a beater respectively, add 15 kg of culture solution dissolved with an inducer, and refine the pulp for 5 minutes. The final particle size of the material is 0.02- 10 μm. Then pour it into a culture tank with a filter membrane, and use a negative pressure of 0.06-0.1 MPa to inhale or a positive pressure of 0.1-0.6 MPa to inhale for homogenate culture every 1 hour, and each culture time is 15 minutes. After culturing for 36 hours, the content of vinca alkaloids in the raw materials was measured by HPLC. The results showed that the content of vinblastine in the raw materials increased by 48%, and the content of vincristine increased by 53%.
[0037] In this example, the formula of culture solution is as follows:
[0038] (1) Standard B5 culture medium;
[0039] (2) The formula of the inducer is:
[0040] Shikimic acid 0.05μmol / L
[0041] Split ...
Embodiment 3
[0053] Divide 50 kg of periwinkle flowers with a water content of 43% for 5 days after collection into 10 equal parts, place them in a beater, add 20 kg of culture solution dissolved with an inducer, and refine the pulp for 5 minutes. The final particle size of the material is 0.02- 10 μm. Then pour it into a culture tank with a filter membrane, use negative pressure 0.06-0.1MPa suction or positive pressure 0.1-0.6MPa air intake to carry out homogenate culture every 1h, and each culture time is 20min. After culturing for 48 hours, the content of vinca alkaloids in the raw materials was measured by HPLC. The results showed that the content of vinblastine in the raw materials increased by 38%, and the content of vincristine increased by 42%.
[0054] In this example, the formula of culture solution is as follows:
[0055] (1) pH=5.8 phosphate buffer solution;
[0056] (2) The formula of the inducer is:
[0057] Shikimic acid 0.05μmol / L
[0058] Split loganin 0.04μmol / L
[0...
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