Arthrodial cartilage proto micro-carrier
A technology of articular cartilage and microcarriers, which is applied in the field of biomedical tissue engineering, can solve the problems of incomplete separation of cells and microcarriers, affect the quality of engineered cartilage, harm to patients' bodies, etc., and achieves low reagent cost, loss avoidance, and simple method. easy effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0024] 1. Take 1 kg of pig fresh articular cartilage, freeze-dry it, pulverize it with a pulverizer, and sieve it to obtain particles with a diameter of 200 μm for later use;
[0025] 2. Add 10 times the volume of 0.25% trypsin to the granules, shake at 37°C for 48 hours, wash with distilled water for 3 times, shake with 1% Triton X-100 at 4°C for 72 hours, and then rinse with distilled water for 48 hours;
[0026] 3. Centrifuge at 1000rpm for 10 minutes, take the precipitate, mix the precipitate with distilled water at a volume of 1:3, put it in a freeze dryer for 12 hours, and pass through 25kGy cobalt 60 Sterilized by irradiation and then digested with 10 times the volume of 0.25% trypsin for 15 minutes, stirred, crushed into granules, washed with distilled water for 3 times, and obtained.
Embodiment 2
[0028] 1. Take 4 kg of fresh bovine articular cartilage, freeze-dry it, pulverize it with a pulverizer, and sieve it to obtain particles with a diameter of 150 μm for later use;
[0029] 2. Add 10 times the volume of 0.25% trypsin (purchased from Sigma Company) to the granules, wash with distilled water for 3 times after shaking at 37°C for 48h, and shake continuously at 4°C for 72h with 1% Triton X-100, then Rinse with distilled water for 48 hours;
[0030] 3. Centrifuge at 1000rpm for 10 minutes, take the precipitate, mix the precipitate with distilled water at a volume of 1:3, put it in a freeze dryer for 12 hours, and pass through 25kGy cobalt 60 Sterilized by irradiation and then digested with 10 times the volume of 0.25% trypsin (purchased from Sigma) for 15 minutes, stirred, crushed into granules, washed with distilled water for 3 times, and obtained.
Embodiment 3
[0032] 1. Take 0.5 kg of fresh articular cartilage, freeze-dry it, pulverize it with a pulverizer, and sieve it to obtain particles with a diameter of 180 μm for later use;
[0033] 2. Add 10 times the volume of 0.25% trypsin (purchased from Sigma Company) to the granules, wash with distilled water for 3 times after shaking at 37°C for 48h, and shake continuously at 4°C for 72h with 1% Triton X-100, then Rinse with distilled water for 48 hours;
[0034] 3. Centrifuge at 1000rpm for 10 minutes, take the precipitate, mix the precipitate with distilled water at a volume of 1:3, put it in a freeze dryer for 12 hours, and pass through 25kGy cobalt 60 Sterilized by irradiation, digested with 0.25% trypsin for 15 minutes, stirred, crushed into granules, washed with distilled water for 3 times, and obtained.
PUM
| Property | Measurement | Unit |
|---|---|---|
| diameter | aaaaa | aaaaa |
| diameter | aaaaa | aaaaa |
| diameter | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More