Polymer composite with internally distributed deposition matter
A deposit, polymer technology, applied in the field of preparation of polymer composites, can solve the problems of unsuitability for commercial processing, difficult to control mixing, insufficient mixing of materials, etc.
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Embodiment 1
[0129] Example 1 - Preparation of polymeric material
[0130] Poly(DL-lactic acid) (Alkermes Medisorb, low I.V. molecular weight = 85 kD, polydispersity = 1.4) was ground to a fine size powder with a pestle and mortar. Alternatively, the poly(DL-lactic acid) is forced out of a carbon dioxide pressurized vessel and through an orifice to form particles. Particulates are recovered from the centrifugal collector, while the carbon dioxide can be recompressed and recycled. The morphology of the particles depends on the antisolvent technique of particle generation from supercritical suspension (PGSS).
[0131] Polymers can also be produced as highly porous monoliths using supercritical fluid processing. In this case, porous scaffolds were prepared in molds prepared with 48-well tissue culture plates (Costar, USA). Weigh 12×100 mg (±1 mg) PLA, add to each well, and seal the mold in an autoclave. The autoclave was heated to 35°C and carbon dioxide was injected for 30 minutes to b...
Embodiment 2
[0132] Example 2 - Addition of biological material - protein
[0133] The protein, in this example avidin (Sigma) labeled with the fluorescent molecule rhodamine, was dissolved in distilled water to form aqueous solutions at concentrations of 1 μg / ml and 10 μg / ml. The liquid can also be selected from those liquids which dissolve biological material but not polymers. 0.5cm 3 Each portion of the protein solution was pipetted onto approximately 250 mg of the polymer sample and then kept in contact with the sample for a period of time (1 second to 48 hours). During this exposure, the liquid was removed by a lyophilization process. We have lyophilized various avidin-rhodamine and ribonuclease solutions (1-μg-250 mg / ml) added to both porous scaffolds and polymer powders within 48 hours. A control scaffold was prepared without protein addition.
[0134] Confocal fluorescence microscopy observations of the material confirmed that the avidin rhodamine was confined to the surface ...
Embodiment 3
[0135] Example 3 - Redistribution of biological material (protein)
[0136] One scaffold from each protein concentration sample in Example 2 was removed from the well as a control. The remaining stent samples were placed in a high-pressure autoclave, heated to 35 ° C, and the same procedure in Example 2 was used in CO 2 Replasticize in. Figure 4 A schematic of plasticization is shown. Confocal fluorescence microscopy of this reprocessed material revealed that the avidin rhodamine was redistributed within the polymer body ( Figure 4 ). Confocal microscopy observations were performed with a Leica TCS4D system (with Leica DMRBE upright fluorescence microscope and Argon-Krypton laser). The red fluorescence of TRITC avidin-rhodamine is excited with a 568nm laser.
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