Method of increasing gas production rate of sewage gas fermentation using xylanase

Abstract

A fermenting method using microbes and enzyme for generating marsh gas with high gas output rate features that the xylanase-type hemi-cellulase is externally added to new marsh gas pool in the ratio of 0.55-0.5 IU / ml (material liquid) and for the normal marsh gas pool, the ratio of xylanase is increased to 5.0 IU / ml. Its advantages are high gas output rate and high content of methane in gas.

Description

Technical field [0001] The invention relates to a biogas fermentation method using microorganisms or enzymes, in particular to a method in which xylanase-based cellulase is added to a biogas fermentation system from an external source to increase the gas production rate. Background technique [0002] One of the problems that always exists in rural semi-continuous and continuous biogas fermentation is that the gas production rate is low, and the actual raw material gas production rate is only about 50% of the theoretical value. One of the important reasons is that hemicellulose, an important component in biogas fermentation raw materials Almost unused. [0003] Hemicellulose accounts for 30% of lignocellulose, and it is generally a highly branched non-crystalline heteropolysaccharide with less than 200 sugar groups, and xylan is the main component of hemicellulose. For example, in the wood of angiosperms (flowering plants), xylan accounts for a large part of its hemicellulos...

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Problems solved by technology

However, the paper is the experimental result of studying the inhibition of enzymes by biogas fermentation liquid outside the anaerobic environment of biogas production; at the same time, xylanase is the general logo of a class of enzymes named internationally EC3.2.1.8 , the xylanases produced by the 8 strains described in the paper are only a few of the two families of F10 and F11 in the large class of xylanases. Therefore, the inferences of the paper have objective limitations

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