Method of increasing gas production rate of sewage gas fermentation using xylanase
A technology of xylanase and biogas fermentation, applied in fermentation, waste fuel, etc., can solve problems such as inference limitations
Inactive Publication Date: 2009-06-17
YUNNAN NORMAL UNIV
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Problems solved by technology
However, the paper is the experimental result of studying the inhibition of enzymes by biogas fermentation liquid outside the anaerobic environment of biogas production; at the same time, xylanase is the general logo of a class of enzymes named internationally EC3.2.1.8 , the xylanases produced by the 8 strains described in the paper are only a few of the two families of F10 and F11 in the large class of xylanases. Therefore, the inferences of the paper have objective limitations
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[0036] (1) The method that xylanase is used for the start-up of new pool
[0037] Start the biogas fermentation according to the conventional biogas fermentation ingredients, and calculate the amount of xylanase added based on the actual volume of fermentation, which is 0.58IU / mL (87BU / mL). In the feeding operation, when the material is fed to 1 / 2 to 2 / 3, the required input xylanase is absorbed and dissolved with water and then poured into the pool, and then the rest of the material is used to seal the pool.
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A fermenting method using microbes and enzyme for generating marsh gas with high gas output rate features that the xylanase-type hemi-cellulase is externally added to new marsh gas pool in the ratio of 0.55-0.5 IU / ml (material liquid) and for the normal marsh gas pool, the ratio of xylanase is increased to 5.0 IU / ml. Its advantages are high gas output rate and high content of methane in gas.
Description
Technical field [0001] The invention relates to a biogas fermentation method using microorganisms or enzymes, in particular to a method in which xylanase-based cellulase is added to a biogas fermentation system from an external source to increase the gas production rate. Background technique [0002] One of the problems that always exists in rural semi-continuous and continuous biogas fermentation is that the gas production rate is low, and the actual raw material gas production rate is only about 50% of the theoretical value. One of the important reasons is that hemicellulose, an important component in biogas fermentation raw materials Almost unused. [0003] Hemicellulose accounts for 30% of lignocellulose, and it is generally a highly branched non-crystalline heteropolysaccharide with less than 200 sugar groups, and xylan is the main component of hemicellulose. For example, in the wood of angiosperms (flowering plants), xylan accounts for a large part of its hemicellulos...
Claims
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IPC IPC(8): C12P5/02
CPCY02E50/343Y02E50/30
Inventor 刘士清张无敌
Owner YUNNAN NORMAL UNIV
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