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Production method of extracellular matrix engineered catheter containing olfactory ensheathing cells

A tissue engineering scaffold and olfactory ensheathing cell technology, which is applied in the field of extracellular matrix tissue engineering scaffold production, can solve the problems of poor histocompatibility, insignificant "bridge" formation, difficult source of seed cells, etc. Effects of stabilizing biological properties

Inactive Publication Date: 2009-07-01
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In view of the current tissue engineering technology in the treatment of spinal cord injury, most of the existing tissue engineering scaffolds are artificially synthesized high molecular polymers, which have poor histocompatibility, difficult sources of seed cells, and inconspicuous "bridge" properties.

Method used

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  • Production method of extracellular matrix engineered catheter containing olfactory ensheathing cells
  • Production method of extracellular matrix engineered catheter containing olfactory ensheathing cells
  • Production method of extracellular matrix engineered catheter containing olfactory ensheathing cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) In vitro culture and purification of olfactory ensheathing cells

[0029] The olfactory area of ​​the nasal mucosa of self-cleaning adult SD rats was isolated and the olfactory mucosa was cut to make a cell suspension. The above cells were cultured by conventional cell culture methods, and the olfactory ensheathing cells were purified by subculture.

[0030] (2) Fabrication of tissue engineering scaffolds and planting of seed cells (olfactory ensheathing cells)

[0031] ① Fabrication of bracket

[0032] Dissolve laminin (final concentration: 10 μg / ml), fibronectin (final concentration: 10 μg / ml) and fibrinogen (final concentration: 100 mg / ml) in common F12 medium containing fetal bovine serum The outer matrix material is made into a compound jelly solution, and then fresh rat plasma is added at a volume ratio of the jelly solution to fresh rat plasma of 20:1, and the jelly solution is immediately sucked into a glass tube with a diameter of 3mm In a few minutes, th...

Embodiment 2

[0040] (1) In vitro culture and purification of olfactory ensheathing cells

[0041] The olfactory area of ​​the nasal mucosa of self-cleaning adult SD rats was isolated and the olfactory mucosa was cut to make a cell suspension. The above cells were cultured by conventional cell culture methods, and the olfactory ensheathing cells were purified by subculture.

[0042] (2) Fabrication of tissue engineering scaffolds and planting of seed cells (olfactory ensheathing cells)

[0043] ① Fabrication of bracket

[0044] Dissolve laminin (final concentration: 15 μg / ml), fibronectin (final concentration: 15 μg / ml) and fibrinogen (final concentration: 150 mg / ml) with common F12 medium containing fetal bovine serum The outer matrix material is made into a compound jelly solution, and then fresh rat plasma is added at a volume ratio of 15:1 between the jelly solution and fresh rat plasma, and the jelly solution is immediately sucked into a glass tube with a diameter of 5 mm In a few mi...

Embodiment 3

[0051] (1) In vitro culture and purification of olfactory ensheathing cells

[0052] The olfactory area of ​​the nasal mucosa of self-cleaning adult SD rats was isolated and the olfactory mucosa was cut to make a cell suspension. The above cells were cultured by conventional cell culture methods, and the olfactory ensheathing cells were purified by subculture.

[0053] (2) Fabrication of tissue engineering scaffolds and planting of seed cells (olfactory ensheathing cells)

[0054] Dissolve laminin (final concentration of 20 μg / ml), fibronectin (final concentration of 20 μg / ml) and fibrinogen (final concentration of 200 mg / ml) in common F12 medium containing fetal bovine serum The outer matrix material is made into a compound jelly solution, and then fresh rat plasma is added at a ratio of 10:1 between the jelly solution and fresh rat plasma, and 200 μl of the jelly solution is dropped into the 24-well culture plate. Then add 20 μl of fresh rat plasma into the hole with the ro...

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Abstract

This invention discloses a method for preparing smell-sheath cell-containing outercell stroma tissue engineering support, which belongs to tissue engineering technical field. It comprises these steps: Use soluted laminin(final concentration is 10-20ª

Description

technical field [0001] The invention belongs to a tissue engineering technology, in particular to a method for manufacturing an extracellular matrix tissue engineering scaffold containing olfactory ensheathing cells. Background technique [0002] Tissue engineering technology has a good clinical application prospect in repairing tissues or organs, and the construction of tissue engineering scaffold is the core content of this technology. But at present, tissue engineering scaffold materials are mainly artificially synthesized polymers and natural materials derived from animals and plants. Chinese patent CN02138129.1 is a method for constructing tissue engineering scaffolds with silkworm silk as a material, and Chinese patent CN02149086.4 is a method for preparing porous chitosan tubes with chitosan, acetic acid, and sodium hydroxide as raw materials. Abroad, people such as Teng wrote "a polymer scaffold with neural stem cells can be Promote functional recovery after spinal...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/08C12N11/02C12N5/079
Inventor 张志坚龚爱华钱雷敏孙湘兰姜平王永忠任春兰端礼荣
Owner JIANGSU UNIV
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