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Xylose oxidation achromatous bacillus SY8 for purifying arsenic contamination and usage thereof

A colorless bacillus, arsenic oxidation technology, applied in the field of environmental microorganisms, to achieve the effect of strong mobility, easy to harm the environment, and reduce the harm of arsenic

Inactive Publication Date: 2009-11-18
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the defects of the existing environmental arsenic pollution purification technology, and obtain a new strain of arsenic oxidizing bacteria, which can oxidize highly toxic trivalent arsenic in the environment to low toxic arsenic pollution

Method used

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  • Xylose oxidation achromatous bacillus SY8 for purifying arsenic contamination and usage thereof
  • Xylose oxidation achromatous bacillus SY8 for purifying arsenic contamination and usage thereof
  • Xylose oxidation achromatous bacillus SY8 for purifying arsenic contamination and usage thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: Isolation of Achromobacter xylosoxidans SY8 from arsenic-contaminated soil

[0024] The experimental soil was taken from the arsenic-contaminated surface soil in Shayang County, Hubei Province, China. The specific operation steps are as follows:

[0025] (1) Sample enrichment: Accurately weigh 100g of soil sample into a 250ml sterilized Erlenmeyer flask, add 5ml of 80mM NaAsO 2 , stir gently and place in a 28°C incubator for one week, pay attention to add sterile water to ensure that the sample is not dry.

[0026] (2) Isolation of arsenic-resistant bacteria: Accurately weigh NaAsO 2 10g of the enriched soil sample was placed in a conical flask filled with 90ml of sterile normal saline, placed in a shaker at 28°C for half an hour, and then 1ml was gradually diluted to 10ml in 9ml of sterile normal saline. -3 , 10 -4 , 10 -5 , respectively take 0.1ml coating containing 800uM NaAsO 2 The CDM solid medium plate was coated with 3 plates for each dilution, an...

Embodiment 2

[0030] Example 2: Arsenic oxidation curve of Achromobacter xylosoxidans SY8

[0031] Pick a single clone of Achromobacter xylosoxidans SY8 and inoculate it into 100ml of CDM liquid medium, and place it in a shaker at 28°C for 48 hours, when the cell density is OD 600 It is about 0.15, stored in a refrigerator at 4°C, and used as a seed bacterial solution for inoculation. Draw 1ml of 1% inoculum into 100ml of fresh CDM liquid medium, place it in a shaker at 28°C for 12 hours, then add NaAsO 2 Mother liquor, so that its final concentration is 800uM, start sampling after continuing to cultivate for 9 hours, and take a sample every 1 hour until NaAsO 2 Until complete oxidation, take 1.2ml and 0.6ml respectively each time, and measure the cell density (OD 600 ) and concentration of pentavalent arsenic (OD 838 ), all are taken and made now. The specific method is as follows: First, measure the cell density (OD 600 ), with deionized water as a reference, directly measure its abs...

Embodiment 3

[0032] Example 3: Oxidation effect of Achromobacter xylosoxidans SY8 on trivalent arsenic in simulated arsenic-polluted water

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Abstract

The invention belongs to the technical field of environmental microorganisms, and in particular relates to a strain of Achromobacter xylosoxidans SY8 capable of oxidizing inorganic trivalent arsenic and its application in purifying arsenic pollution. The present invention is characterized in that an achromobacter xylosoxidans SY8 strain capable of oxidizing inorganic trivalent arsenic is isolated from arsenic-polluted soil. The strain can oxidize the inorganic trivalent arsenic in the arsenic-polluted environment to the inorganic pentavalent arsenic, which greatly reduces the toxicity of the arsenic in the environment and enhances the adsorption and removal of the arsenic. The bacterial strain of the present invention is named Achromobacter xylosoxidans SY8 (Achromobacter xylosoxidans), is a newly discovered arsenic oxidizing bacterium, and its preservation number is CCTCC NO: M207048. Preliminary research shows that the bacterial strain of the present invention has good application prospects in controlling arsenic pollution in the environment.

Description

technical field [0001] The invention belongs to the technical field of environmental microorganisms, and in particular relates to the screening of an Achromobacter xylosoxidans SY8 strain capable of oxidizing inorganic trivalent arsenic and its use in purifying arsenic pollution. Background technique [0002] At present, heavy metal pollution has become a global problem, especially arsenic pollution. World Health Organization officials announced that at least 50 million people in the world are facing the threat of endemic arsenic poisoning, most of them are Asian countries, and China is also one of the countries most seriously affected by arsenic poisoning. Although arsenic pollution in China has geological factors, human factors are more obvious. For example, mining and smelting of ores, processing of arsenic products, and burning of coal have seriously polluted soil, groundwater, and rivers. [0003] Aiming at arsenic pollution, there are currently some treatment methods ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C02F1/58B09C1/10A62D3/00C12R1/025
Inventor 王革娇蔡林
Owner HUAZHONG AGRI UNIV
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