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Injection medicine composite containing salvianolic acid A and its preparation

A technology for salvianolic acid and composition of salvia miltiorrhiza is applied to the injection pharmaceutical composition containing salvianolic acid A and the field of preparation thereof, and can solve the problems of inability to obtain bulk grade salvianolic acid A and the like.

Inactive Publication Date: 2010-04-14
PHARMA RES INST OF BENCAO TIANYUAN OF BEIJING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] After reviewing the literature and patents, there is no report on the compatibility of salvianolic acid A and breviscapine, but there is a report on the compatibility of salvianolic acid A and breviscapine. Although this drug combination has a certain curative effect, due to the existence of the existing technology There is a certain defect: the salvianolic acid obtained is mainly salvianolic acid B, and the batch-grade salvianolic acid A cannot be obtained. Therefore, the batch-grade salvianolic acid A and brevis Whether the flower elements can be combined requires researchers to go through a large number of scientific research experiments to determine

Method used

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  • Injection medicine composite containing salvianolic acid A and its preparation
  • Injection medicine composite containing salvianolic acid A and its preparation
  • Injection medicine composite containing salvianolic acid A and its preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0201] Extraction and purification of salvianolic acid A:

[0202] Extract the salvia miltiorrhiza with 50% ethanol solution to obtain the alcohol extract, concentrate the ethanol to the utmost, adjust the pH value to 8.0, heat at 50°C for 4 hours, filter the solution, and separate the filtrate by HPD-300 macroporous resin column chromatography , first eluted with water and 20% dilute ethanol to remove impurities, then eluted with 50% ethanol, collected the eluate, and recovered the ethanol to the utmost; the concentrated solution was separated by polyamide chromatography column, first water, 35% ethanol The solution was eluted, the eluent was discarded, and then eluted with 75% ethanol solution, and the ethanol was recovered to the limit; the concentrated solution was adjusted to a pH value of 2, extracted with an organic solvent ethyl acetate, and the organic solvent phase was separated to obtain a drug-containing solution. Concentrate, dry or freeze-dry to obtain salvianoli...

Embodiment 2

[0215] Extraction and purification of salvianolic acid A:

[0216] Extract Salvia miltiorrhiza with 80% ethanol solution to obtain an alcohol extract, concentrate the ethanol to the utmost extent, adjust the pH value to 6.0, 130°C temperature, and a gauge pressure of 0.17MPa, and heat for 6 hours; the solution is filtered, and the filtrate is passed through HPD-450 Pore ​​resin column chromatography separation, first eluted with water and 30% dilute ethanol to remove impurities, then eluted with 70% ethanol, collected the eluate, recovered ethanol to the end; the concentrated solution was used with Sephadex LH- 20 Chromatographic column separation, first eluted with water and 50% ethanol solution, discarded the eluent, and then eluted with 95% ethanol solution, recovered the ethanol to the utmost; extracting and separating the organic solvent phase to obtain a drug-containing solution, concentrating, drying or freeze-drying to obtain salvianolic acid A, and the content of salv...

Embodiment 3

[0229] Extraction and purification of salvianolic acid A:

[0230] Salvia miltiorrhiza is extracted with 65% ethanol solution to obtain an alcohol extract, and the alcohol extract is concentrated to ethanol to the utmost, and the pH value is adjusted to 5.5, 115° C. temperature, and a gauge pressure of 0.07 MPa, and heated for 3 hours; the solution is filtered, and the filtrate is filtered through 1300-1 Pore ​​resin column chromatography separation, first eluted with water and 25% dilute ethanol to remove impurities, then eluted with 65% ethanol, collected the eluate, recovered ethanol to the end; the concentrated solution was used with Sephadex LH- 20 Chromatographic column separation, first eluted with water and 45% ethanol solution, discarded the eluent, and then eluted with 90% ethanol solution, recovered ethanol to the utmost; Extract and separate the organic solvent phase to obtain a drug-containing solution, concentrate, dry or freeze-dry to obtain salvianolic acid A w...

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Abstract

The present invention discloses one kind of injection medicine composition containing red sage salvianolic acid A and its preparation process. The injection medicine composition has the composition determined through pharmacodynamic experiment, pharmacokinetic experiment and toxicologic experiment as red sage salvianolic acid 1-10 weight portions and breviscapine 1-20 weight portions, preferably red sage salvianolic acid 1-5 weight portions and breviscapine 1-12 weight portions. The injection medicine composition may be prepared into injection, infusion solution or powder for injection. Pharmacologic experiment shows its excellent pharmacologic effect.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine pharmacy, in particular to an injection pharmaceutical composition containing salvianolic acid A and a preparation method thereof. Background technique [0002] Salvianolic acid A [salvianolic acidA] is the most active ingredient in Danshen, which has good pharmacological effects (Du Guanhua [Basic Medicine and Clinic, 2000, 20(5): 10-14], Hu Yiyang [Acta Pharmacology of Traditional Chinese Medicine, 1997 , 18(5):478-480]), the content of salvianolic acid A in Salvia miltiorrhiza is very low, only about 5 / 10,000, even if it is extracted through a series of processes, only trace amounts of salvianol can be obtained Therefore, traditional Chinese medicine uses Danshen to participate in the compatibility of other medicinal materials, and its essence is the compatibility with other relatively weak components of Danshen (such as tanshinone, salvianolic acid B, etc.). However, the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/7048A61P9/00G01N30/02A61K31/343
Inventor 顾群栗艳彬林治荣鄯慧珍米长江李志刚渠守峰
Owner PHARMA RES INST OF BENCAO TIANYUAN OF BEIJING
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