Use of ulinastatin in preparing medicine for treating and/or preventing spinal cord injury

A technology for ulinastatin and spinal cord injury, which is applied in the new medical application of ulinastatin and the application field of preparation of drugs for the treatment and/or prevention of spinal cord injury, which can solve the problem of lack of treatment and/or preventive drugs for spinal cord injury , to achieve significant therapeutic and/or prophylactic effects

Inactive Publication Date: 2007-08-22
GUANGDONG TECHPOOL BIO-PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Contemporary medicine still lacks effective treatment and / or prevention drugs for spinal cord injury

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 Preparation of Ulinastatin Dry Powder for Injection

[0019] Take 500 million units of filtered sterilized ulinastatin aqueous solution, add 100 grams of mannitol to dissolve, adjust the pH to neutral, add water for injection to 10,000 ml, add sodium chloride to adjust isotonicity, filter aseptically, and pack in 5,000 ml In a vial, freeze-dried under sterile conditions, that is.

Embodiment 2

[0020] Example 2 Preparation of Ulinastatin Injection

[0021] Take 500 million units of filtered sterilized ulinastatin aqueous solution, add 100 grams of mannitol to dissolve, adjust the pH to neutral, add water for injection to 10,000 ml, add sodium chloride to adjust isotonicity, filter aseptically, and pack in 5,000 ml Get it in a vial.

Embodiment 3

[0022] Example 3 Therapeutic Effects of Ulinastatin on Rats with Spinal Cord Injury Caused by Experimental Shock

[0023] Animal model and grouping: 60 SD rats, weighing 260g-300g, male or female. The spinal cord injury rat model was made by the following method: intraperitoneal anesthesia with 2% pentobarbital sodium 40mg / kg. The limbs of the rats were fixed in the prone position, and a posterior midline incision was made on the back. Cut the skin and subcutaneous tissue, expose and bite off the T 10 ~T 12 Laminae and spinous processes. The spinal cord was injured by the modified Allen's blow method. The spinal cord was hit by gravity with a homemade 10g hammer under the guidance of a glass catheter (the intensity was 10g×2.5cm). After waking up from anesthesia, the lower limbs were flaccidly paralyzed. Three days after the operation, 200,000 u / kg of penicillin was intramuscularly injected twice a day, and the rats were artificially defecated twice a day.

[0024] Ulina...

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PUM

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Abstract

The present invention relates to the application of ulinastatin in preparing medicine for preventing and treating spinal cord injury. Ulinastatin has obvious spinal cord injury preventing and treating effect. The medicine is prepared into injection or freeze dried powder for injection.

Description

technical field [0001] The present invention relates to the field of medicinal chemistry. Specifically, the present invention relates to a new medical application of ulinastatin, that is, the application of ulinastatin in the preparation of medicines for treating and / or preventing spinal cord injury. Background technique [0002] Ulinastatin, also known as Human Urinary Trypsin Inhibitor (Human Urinary Trypsin Inhibitor), is a glycoprotein isolated and purified from human urine, consisting of 143 amino acids, with a molecular weight of 40-50KD as determined by SDS-PAGE. With an isoelectric point of 2.6, it is a heat-stable acidic protein. Ulinastatin is a typical Kuniz-type protease inhibitor with two active functional areas. [0003] Ulinastatin can effectively inhibit a variety of hydrolytic enzymes, and has a significant stabilizing effect on cell membranes and lysosome membranes. Ulinastatin is not only an enzyme inhibitor in the human body, but also an important physi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/57A61K9/19A61P19/00A61P25/00
Inventor 傅和亮侯永敏吴蓉蓉王晓岩雷瑶
Owner GUANGDONG TECHPOOL BIO-PHARMA CO LTD
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